水稻萌发期耐Cu^(2+)胁迫的QTL定位  

QTL analysis on copper-resistant at germination stage in rice(Oryza sativa L.)

作　　者：饶玉春[1,2] 杨窑龙[2] 李晓静[1] 马伯军[1] 曾大力[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006

出　　处：《浙江师范大学学报（自然科学版）》2013年第2期198-204,共7页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家自然科学基金资助项目(31201183);浙江省教育厅项目(Y201225687);现代农业生物技术与作物病害防控浙江省重中之重学科开放基金资助项目(ZC323012033;ZC323012046)

摘　　要：以典型籼粳交(春江06/台中本地1号)双单倍体(DH)群体为材料,经100μmol/L CuSO4溶液对其双亲及DH群体进行处理,考察了DH群体及其双亲的耐铜性,并利用业已构建的分子连锁图谱进行了数量性状基因座(QTL)区间分析.共检测到23个QTLs,其中与铜胁迫有关的QTL有7个,分别位于水稻第1,2,3和7染色体上;贡献率最大的QTL为qTGR-2,变异解释率为14.60%,其增效等位基因来自台中本地1号;芽质量的QTL qTSW-1增效等位基因来自春江06;余下的几个耐铜胁迫的QTL增效基因均来自台中本地1号.同时,在3号染色体上检测到了一个QTL,可显示发芽率受铜胁迫抑制的程度.A rice double haploid（DH） population,derived from a typical Indica-japonica cross（CJ06/TN1） F1 population via the anther culture,and its parents were treated with 100 μmol/L CuSO4,respectively.By analyzing copper resistance in DH population and its parents,mapping of quantitative trait loci（QTL） using an available molecular linkage map,twenty-three QTLs were detected,seven of which were related to the copper resistance and localized on rice chromosome 1,2,3 and 7,respectively.Among them,one QTL with the greatest contribution（14.60%） was qTGR-2.One additive allele was originated from TN1,whereas another additive allele related to shoot weight,qTSW-2 was resulted from CJ06.The remaining additive alleles were derived from TN1.Meanwhile,it was detected a QTL on chromosome 3,which demonstrated the degree of inhibition of copper stress affected on germination rate.

关 键 词：水稻 萌发期 铜毒害 数量性状基因座 

分 类 号：Q37[生物学—遗传学]