p53诱导死亡结构域蛋白在大鼠创伤性脑损伤中的表达变化  

Expression profile of PIDD in rat brain after traumatic brain injury

作　　者：万春华[1] 毛辉[2] 陈建[2] 赵建亚[1] 姜俊康[1] 

机构地区：[1]南通大学公共卫生学院营养与食品卫生教研室,江苏226001 [2]南通大学附属医院神经外科

出　　处：《交通医学》2013年第2期115-117,共3页Medical Journal of Communications

基　　金：国家自然科学基金资助项目(31071288);南通市科技项目(BK2012090)

摘　　要：目的:探索创伤性脑损伤病理过程中p53诱导死亡结构域蛋白(PIDD)的表达变化。方法:建立大鼠脑损伤模型,采用免疫印迹法和免疫组织化学法检测损伤后的大鼠皮层组织中PIDD的表达和定位变化。结果:(1)在假手术脑组织中,PIDD表达很低,脑损伤后其表达逐渐增加,在3天左右达到最高峰,之后表达下降;(2)免疫组织化学法和免疫荧光实验表明,PIDD在损伤后的脑组织中表达明显上升,并且主要定位于神经元之中。Objective： To explore the expression profile of PIDD in rat traumatic brain injury. Methods： The model for traumatic brain injury was established in adult rats, and the expression and distribution of PIDD following brain trauma was analyzed using western blot, immunohistochemistry and immunofluorescence assays. Results： The expression of PIDD was gradually increased, reached a peak on the 3rd day, and then decreased afterward in rat brain cortex following injury. PIDD was mainly distributed in neurons. Conclusion： The expression of PIDD was upregulated in rat brain cortex following injury, suggesting that PIDD might be involved in the pathological process of traumatic brain injury.

关 键 词：创伤 性脑损伤 p53诱导的死亡结构域蛋白 大鼠 神经元 免疫组织化学法 

分 类 号：R651.15[医药卫生—外科学]