大鼠改良Ono式腹部异位心脏移植模型观察心肌内心电图  

Using Modified Ono’s Heterotopic Heart Transplantation Modle of Rat to Record IMEG

作　　者：史加海[1] 韩杰[2] 贾一新[2] 杨敦鹏[1] 李阳成[1] 杨学超[1] 刘俊华[1] 孟旭[2] 

机构地区：[1]南通大学附属医院心胸外科,江苏226001 [2]首都医科大学附属北京安贞医院

出　　处：《交通医学》2013年第2期125-128,133,共5页Medical Journal of Communications

基　　金：南通市应用研究计划项目(K2010055);南通大学附属医院博士启动基金

摘　　要：目的:建立稳定的、能描记自主和起搏心肌内心电图(intramyocardial electrogram,IMEG)的大鼠心脏移植模型。方法:Lewis健康雄性大鼠为受体,分别用lewis、SD健康雄性大鼠为供体,用改良Ono法行同基因移植(Lewis-Lewis)和异基因移植(SD-Lewis)。通过供心表面埋植的临时起搏导线,PCLAB-UE记录自主的心肌内心电图和起搏心肌内心电图。结果:预实验模型建立20只成功11只(55%);正式实验模型建立52只成功46只(88.46%),其中SD-Lewis34例,Lewis-Lewis12例。全部大鼠通过"隧道法"成功埋植3根起搏导线,能获得稳定的心肌内心电图(含自主和起搏的)。两组间在成功率、供心摘取时间、血管吻合时间、手术总需时间等方面比较差异有统计学意义(P<0.05)。结论:建立大鼠改良Ono式腹部异位心脏移植模型、并通过改良"隧道法"方法埋植多个心肌内电极,效果可靠、稳定,获得满意的自主和心室起搏心肌内心电图。Objective：This research aimed to establish stable rat heart transplantation models with which IMEGs can be observed. Methods：Syngeneic heart ransplantation models （Lewis to Lewis） and allogeneic heart transplantation models （（Lewis to SD） were established with health male Lewis and SD rats by modified Ono＇s method. Autonomous IMEGs（in- tramyocardial electrogram ,IMEG） and VERs （ventricular evoked response ,VER）were recorded with epicardiac pacing leads fixed at right ventricular outflow tracts, left ventricular apex and free wall. Results：The achievement ratio in the pre- liminary experiment and the formal experiment had a significent difference （P〈0.05）： 55%（11/20） VS 88.46%（56/52）. The time of removing donor＇s hearts, anastomosing vessels, ischemia and the total operatioin time were statistically different in the two groups. 3 epicardiac pacing leads were implanted in each recipient of the sucessful models （SD to Lewis：34; Lewis to Lewis：12） in each through a ＂tunnel＇s way＂. IMEGs were recorded successfully.Conclusions：The modified Ono＇s het- erotopic heart transplantation modle of rat implanted epicardiac pacing leads through a ＂tunnel＇s way＂ is reliable and sta- ble. Autonomous IMEGs and VERs can be successfully recorded through this kind of modle.

关 键 词：心脏移植 心肌内心电图 大鼠 

分 类 号：R392.4[医药卫生—免疫学]