血清低密度脂蛋白胆固醇匀相测定法试剂的研制  

Homogeneous method for directly measuring serum low-density lipoprotein cholesterol

作　　者：张世俊[1] 蒋知新 张金树 高德路 李培瑛[2] 

机构地区：[1]解放军第三○五医院检验科,北京100017 [2]卫生部北京老年医学研究所

出　　处：《中华检验医学杂志》2000年第5期276-279,共4页Chinese Journal of Laboratory Medicine

基　　金：全军"九五"第二批科研基金! (98M15 9)

摘　　要：目的　研制低密度脂蛋白胆固醇 (LDL C)直接法测定的试剂。方法　低密度脂蛋白(LDL)与聚阴离子和表面活性剂Ⅰ形成稳定复合物 ,而非LDL则在缺乏偶联剂条件下被胆固醇酶试剂消除 ,在表面活性剂Ⅱ和色原作用下 ,LDL释放胆固醇并显色。结果　本室研制的试剂 (Y)与聚乙烯硫酸盐 (PVS)沉淀法 (X1)、Friedewald公式计算 (X2 )和日本第一化学试剂 (X3)相关良好 ,分别为Y =1.0 848X1- 0 .2 398;Y =1.0 0 42X2 - 0 .0 4778;Y =1.0 5 98X3- 0 .10 6 7,在LDL C浓度为 2 9.0 6mmol/L以下线性良好 ,r =0 .9998,LDL C低、中、高值血清标本的批内和批间变异系数 (CV)分别为 1.49%、0 .6 8%、0 .40 %和 2 .2 1%、1.41%、0 .81% ,平均回收率为 97% ,胆红素、血红蛋白等干扰不明显。结论自制LDL C直接测定法试剂测定结果的准确度和精密度均符合临床要求。Objectives To study direct assay reagents for measuring low density lipoprotein cholesterol.Methods Low density lipoprotein was combined with polyanion and detergentⅠ. Cholesterol esterase, cholesterol oxides, and subsequently peroxidase in a non coupler forming reaction eliminated the non LDL compositions. The combined LDL was released by the detergent Ⅱ; the enzyme reaction with LDL C in the presence of the coupler produced color in proportional to the amount of LDL cholesterol in the sample.Results The directly determined LDL C values by home prepared reagent( Y ) were closely correlated with those obtainod by precipitation with PVS( X 1) ( Y =1.084 8 X 1-0.239 8) and F formula( X 2)( Y =1.004 2 X 2-0.047 78) and Japanese reagent ( X 3)( Y =1.059 8 X 3-0.106 7).The assay was linear up to at least 29.06mmol/L ( r = 0.999 8). The inter and intra CV at low, middle, high concentration specimens were 1.49%, 0.68%, 0.40%; and 2.21%, 1.41%, 0.81% respectively,the mean recovery rate was 97%.Interference with bilirubin and hemoglobin was neglected.Conclusion The precision and accuracy of LDL C determination with this direct assay method can meet the clinical demand.

关 键 词：低密度脂蛋白胆固醇 匀相测定法 血清诊断 

分 类 号：R446.1[医药卫生—诊断学]