沼泽红假单胞菌的分离鉴定、纯化及计数培养基优化研究  

The research on isolation, identification and purification, and optimization of counting medium component of Rhodopseudomonas palustris

作　　者：欧小兵[1] 全亚玲[1] 戴静[1] 李涵依[1] 车晨[1] 郝葆青[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《西南民族大学学报（自然科学版）》2013年第3期322-326,共5页Journal of Southwest Minzu University(Natural Science Edition)

摘　　要：目的:通过分离鉴、纯化与鉴定菌株,然后按沼泽红假单胞菌所需的营养组分,优化计数培养基的配方,为生产中的计数提供试验数据.方法:待分离的菌落接种于培养基,然后进行反复的纯化,最后观察菌落的形态,按照细菌鉴定手册鉴定;按所需碳源、氮源、磷酸盐、酵母膏等因素进进行正交设计,优化出最佳的培养基配方.结论:分离与纯化得到一株红红螺菌科沼泽红假单胞菌;通过优化得到最优及验证试验得到最佳的计数培养基配方为:氯化铵1.5 g/L,乙酸钠1.5 g/L磷酸氢二钾0.4g/L,氯化钠1.0g/L,硫酸镁0.3g/L,酵母膏2.0g/L,琼脂14.0g/L.该配方能够对沼泽红假单胞菌准确及简捷的计数.Objective： By separating, purifying and identifying authentication strains, and then optimizing counting medium component according to swamp red pseudomonas with the nutrition component, to provides counting experimental data for production. Method： Separating strain vaccination in medium, then repeatedly purifying, and finally observing the character of the strain according to the bacteria identification manual; conducting orthogonal design according to the carbon source, nitrogen source, phosphate, and yeast extract factors to optimize the medium component. Conclusion： Through separation and purification, a plant of red rhodospirillaceae swamp with red pseudomonas is obtained; Through optimization and verification test, the best counting medium formula is proved to be： ammonium chloride 1.5g, sodium acetate 1.5 g, dipotassium hydrogen phosphate 0.4 g and Lsodium chloride 1.0 g, magnesium sulfate 0.3 g, yeast extract 2.0 g, agarl4g. This medium is accurate and convenient in counting the number of bacterium Rhodopseudomonas palustris.

关 键 词：沼泽红假单胞菌 光合菌 分离与纯化 

分 类 号：S182[农业科学—农业基础科学]