无偿献血者ALT检测与乙型肝炎关系的分析  

Analysis of the relationship between ALT and hepatitis B of blood donors

作　　者：漆萍[1] 钟方才[2] 万小涛[2] 邓文英[1] 吴垚林 

机构地区：[1]内江市中心血站,四川内江641000 [2]内江市第一人民医院,四川内江641000

出　　处：《实验与检验医学》2013年第2期183-184,共2页Experimental and Laboratory Medicine

摘　　要：目的探讨无偿献血者血液筛查ALT检测与乙型肝炎的关系,以及其在血液筛查中的意义。方法对本站2012年7-8月体格检查合格的2915份无偿献血者标本进行ALT速率法检测。对于ALT>40U/L的标本,采用酶联免疫吸附法(ELISA)检测HBsAg,并通过实时荧光定量PCR法检测HBV-DNA。结果结果显示,2915例无偿献血标本中,共检测出ALT>40U/L的标本共318例,占献血人数的10.91%。对此318例标本进行HBsAg、HBV-DNA检测,得出阳性结果共5例,仅占318例样本的1.57%。结论ALT>40U/L无偿献血者乙型肝炎检出仅占极少部分,大部分为其它原因引起ALT异常。Objective To explore the relationship of ALT and hepatitis B of blood donors, as well as its significance in blood screening. Methods ALT of 2915 specimens from July to August 2012 were detected using velocity method. For the specimens with ALT 〉 40U/L, the HBsAg was measured by enzyme-linked immunosorbent assay （ELISA）, and HBV-DNA was measured by real-time quantitative PCR. Results Among these 2915 specimens, 318 specimens＇ ALT were more than 40U/L, accounting for 10.91%. Further study revealed that, among these 318 specimens, there were 5 HBsAg or/and HBV-DNA positive cases, accounting for 1.57%. Conclusion Hepatitis B-positive blood makes up a very small proportion of the specimens with ALT 〉 40U/L. A proportion of donating blood has been treated as unqualified blood because of the ALT 〉40U/L criterion. We suggest that HBV- DNA detection should be used for blood quality estimating.

关 键 词：无偿献血 ALT 不合格率 

分 类 号：R446.112[医药卫生—诊断学] R457.12[医药卫生—临床医学]