干旱胁迫下毛竹根尖Ca^(2+)分布及外源Ca^(2+)作用机制  

Distribution of Ca^(2+) at the Tip of Phyllostachys edulis Root under Drought Stress and Physiological Functions of Exogenous Ca^(2+)

作　　者：应叶青[1,2] 杜旭华[3] 姜琴[2] 徐川梅[2] 吴家胜[2] 

机构地区：[1]北京林业大学,北京100083 [2]浙江农林大学亚热带森林培育国家重点实验室培育基地,临安311300 [3]国家林业局竹子研究开发中心,杭州310012

出　　处：《林业科学》2013年第4期141-146,共6页Scientia Silvae Sinicae

基　　金：浙江省科技厅重点项目(2009C12089;2012T201-01)

摘　　要：Ca2＋是植物细胞中重要的信号物质,参与许多逆境下生理信号的转导。植物干旱胁迫下产生的Ca2＋信号,可通过与钙调蛋白等钙受体结合,放大信号并进行震荡传递,以此调节气孔关闭及活性氧的产生（Knightetal.,1997;Shinozaki,1997;宗会等,2001）,作出抵御逆境的有利反应。Drought is one of the main disastrous weather factors. In this study, we investigated the distributing character of Ca2＋ in Phyllostachys eduli root tip and the influence mechanism of exogenous Ca2＋ and its inhibitor under drought stress, by using the laser scanning confocal microscope （LSCM）, to explore how the calcium signaling play its role under drought stress. The results showed that calcium ions in apical root cap and elongation zone were more than that in other parts. The total content of intracellular Ca2＋ increased in about 15 min under treatment with high PEG concentration. The Ca2＋ accumulated in the cytoplasmic under the high PEG concentration stress, while the accumulation was not obvious under low PEG concentrating stress. The relative electric conductivity （REC）, malondialdehyde （MDA） content, catalase （CAT） activity and superoxide dismutase （SOD） activity in Ph. eduli treated by drought stress increased with the treating time. But the POD activity increased firstly and then declined with the time. When the exogenous Ca2＋ were added, REC and MDA content reduced significantly compared to the control. And the antioxidant enzyme activity gone up, such as POD, SOD and CAT activity. Calcium signaling inhibitors, such as EGTA,heparin,LaCl3 and CPZ, were able to block conducting system. When the inhibitors were added, REC and MDA contents in the bamboo leaves increased obviously, and the CAT and SOD activities were reduced significantly. These inhibitors, except for EGTA, also cause the POD activity to obviously reduce compared to the control. It was concluded that exogenous Ca2＋ can promote the resistance of Ph. eduli to draught stress by regulating activities of the protective enzymes.

关 键 词：毛竹 干旱胁迫 Ca2+信号 激光共聚焦 抗氧化保护酶 

分 类 号：S718.43[农业科学—林学]