α-蒎烯对大麦虫体内解毒酶活性的影响  

Effects of α-pinene Fumigation on Detoxifying Enzymes Activities of Zophobas morio

作　　者：田雨浓[1] 马伟[2] 韦庆慧[1] 韩旭洋[1] 罗帅[1] 陈旭日[1] 邱本军[1] 马玲[1] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《林业科学》2013年第4期152-156,共5页Scientia Silvae Sinicae

基　　金：国家林业局林业科学技术推广项目2011(48);黑龙江省科技攻关项目(GA09B203-1)

摘　　要：当今人们的环保意识不断提高,寻找替代化学农药进行有害生物综合防治成为当今各国科研工作者关注的热门话题（Isman,2008;Rajendran et al.,2008）。随着精油类农药商品化的成功,这必将大力促进此类植物源农药的研制,可以预见植物精油用于植物病虫害的防治将是今后的发展趋势之一（袁海滨等,2007）。In order to study the insecticidal activity and toxicity mechanism of α-pinene（a plant volatile organic compound）, we tested the acute toxicity of α-pinene against 4th instar larvae of Zophobas morio in a gas-fumigation system, and investigated the activities of carboxylesterase （CarE）, acid phosphatase （ACP）, alkaline phosphatase （ALP） and the glutathione S-transfer （GSTs）in the larvae at different fumigation time. The results showed that the acute toxicity LC50 and LC20 values of α-pinene against the 4th instar larvae in 48 h were 21.435 and 12.140 μg·L-1, respectively. Under the fumigation with LC50 and LC20 concentrations （21.435 and 12.140 μg·L-1） of α-pinene, the activity of CarE was significantly inhibited. The activities of ALP and GSTs first increased but subsequently reduced. Under the fumigation at LC20 concentration of α-pinene, the activities of ACP was increase. Under the fumigation at LC20 concentrations of α-pinene, the ACP activity showed first increased but subsequently reduced. It could be inferred from the above results that α-pinene had a high biological activity on Z. morio, which could effectively interference the detoxifying enzymes, disrupt the normal physiological metabolism and show high toxicity effect on the insect.

关 键 词：Α-蒎烯 大麦虫 急性毒性 解毒酶 

分 类 号：S718.7[农业科学—林学]