日本囊对虾apo-14基因的全克隆及序列分析  

Cloning and Sequence Analysis of the apo-14 Gene in Marsupenaeus japonicus

作　　者：乔莹[1] 王军[1] 钟声平[1] 刘洪涛[1] 毛勇[1] 

机构地区：[1]厦门大学海洋与地球学院,福建厦门361005

出　　处：《热带生物学报》2013年第1期17-24,共8页Journal of Tropical Biology

基　　金：国家虾产业技术体系岗位专家项目:"日本囊对虾育种"(CARS-47)

摘　　要：以日本囊对虾(Marsupenaeus japonicus)肝胰腺组织为实验材料,提取总RNA,通过3'以及5'-RACE基因克隆技术,首次在日本囊对虾中克隆到载脂蛋白apolipoprotein-14(apo-14)基因的全长序列,包含完整的ORF以及3'-UTR和5'-UTR。日本囊对虾apo-14cDNA序列全长为784bp,其中ORF长度为420bp,编码139个氨基酸。对虾apo-14氨基酸序列与其他鱼类apo-14氨基酸序列有较高的相似性。Full length of apolipoprotein-14 （apo-14） gene was cloned from the total RNA of Marsupenaeus japonicus. Sequence analysis showed that the full cDNA sequence was 784 bp in length including the coding region of 420 bp and encoded 139 amino acids. Homology analysis of the apolipoprotein-14 amino acid sequence indicated high sequence similarity in apo-14 amino acid sequences between M. japonicus and other fish.

关 键 词：日本囊对虾 载脂蛋白 apo-14 克隆 序列分析 

分 类 号：Q781[生物学—分子生物学]