AtSIAK在植物抗盐胁迫响应中的功能研究  

Functional Analysis of AtSIAK Gene in Response to Salt Stress in Arabidopsis

作　　者：刘明[1] 杨浪[1] 阳成伟[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2013年第3期86-90,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871337,30900789);广东省高等院校珠江学者岗位计划资助项目(2010年度)

摘　　要：AtSIAK基因在拟南芥中编码ABC1类蛋白激酶,RT-PCR检测AtSIAK基因在拟南芥的根、茎、叶、花、角果等组织中均有表达,与利用AtSIAK基因的启动子驱动GUS报告基因的研究结果一致.AtSIAK基因受不同胁迫(MV和NaCl)和激素ABA、SA处理均有明显的上调表达,其中在NaCl处理下最明显.不同浓度NaCl处理使35S::AtSIAK过表达植株比野生型(Columbia)和siak1突变体的种子萌发率和子叶变绿率高,表明AtSIAK基因参与抗盐胁迫的响应.We have cloned a AtSIAK （Salt Induction ABCl-like Kinase） gene, encoded an ABCl -like protein kinase in Aradidopsis thaliana, the expression patterns of AtSIAK in Aradidopsis were detected by RT-PCR and GUS staining. AtSIAK expressed in all tissues including root, leaf, silique and seedling, and it expressed most in leaves. The transcription level of the AtSIAK gene was analyzed by semi-quantitive RT-PCR in ABA, SA, MV and NaCl stress conditions, the transcription level was up-regulated, especially in the 0. 3 mol/L NaCl stress. Moreover, 35S- AtSIAK over expresssion plants had higher rate of cotyledon greening and long primary root length than siak-1 mutant and Col-O after the treatment of NaCI. These results suggested that AtSIAK is involved in salt stress response.

关 键 词：拟南芥 ABC1类蛋白激酶 AtSIAK 盐胁迫 

分 类 号：Q291[生物学—细胞生物学]