黄花烟草授粉前后雌蕊生理生化变化研究  

Physiological and Biochemical Changes in Pistil of Nicotiana Rustica L. before and after Pollination

作　　者：康洪梅[1] 廖菊够[1] 龙春瑞[1] 代瑾然[1] 马文广[2] 索文龙[2] 姚恒[2] 王建光[1] 陈穗云[1] 

机构地区：[1]云南大学生命科学学院,云南昆明650091 [2]云南省烟草农业科学研究院,云南玉溪653100

出　　处：《华南师范大学学报（自然科学版）》2013年第3期95-98,共4页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31101416);云南省烟草公司项目(2011YN09)

摘　　要：以黄花烟草(Nicotiana rustica L.)为材料,研究其柱头和花柱在授粉前后可溶性糖、可溶性蛋白、酚类、氨基酸、脂肪含量及过氧化物酶(POD)活性的变化.结果表明:授粉后柱头和花柱中的可溶性糖含量和POD活性均降低,可溶性蛋白和脂肪含量增加,总酚含量无明显变化,柱头氨基酸含量降低,而花柱氨基酸含量增加;无论是授粉前还是授粉后,与同一时期柱头比较,花柱中的可溶性糖含量高,可溶性蛋白、酚类、氨基酸、脂肪的含量及POD活性都低.Variation of soluble sugar, protein, total phenolics, amino acid, fat contents and POD activity in pistil of Nicotiana rustica L. before and after pollination were studied. The results showed that soluble sugar content and POD activity of Nicotiana rustica L. both in stigma and style were lower before pollination than after pollination, and the soluble protein and fat contents in the pistil were higher before pollination than after pollination, the total phenolics content in pistl was unchanged before and after pollination, and the amino acid content of stigma was higher before pollination than after pollination, but the amino acid content of style was lower before pollination than after pollination; Whether before or after pollination, the soluble sugar content was higher in style than in stigma; and soluble protein, total phenolics, amino acid, fat contents and POD activity were higher in stigma than in style.

关 键 词：黄花烟草 授粉 生理 柱头 花柱 

分 类 号：Q945.63[生物学—植物学]