海绵共附生真菌Hansfordia sp.次级代谢产物的分离与鉴定  

Secondary metabolites from marine sponge-associated fungus Hansfordia sp.

作　　者：赵丹奇[1,2] 吴泽宏[2] 刘东[2] 裴月湖[1] 林文翰[2] 白皎[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]北京大学天然药物及仿生药物国家重点实验室,北京100191

出　　处：《沈阳药科大学学报》2013年第5期342-345,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家科技重大专项基金资助项目(2009ZX09501-014)

摘　　要：目的从海绵共附生真菌Hansfordia sp.的次级代谢产物中得到具有活性的化合物。方法采用硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC色谱分离纯化,根据化合物的NMR、MS波谱数据和理化性质进行结构鉴定。结果从真菌Hansfordia sp.的固体发酵物的乙酸乙酯提取物中分离得到8个单体化合物,分别鉴定为1,3,6-trihydroxy-8-methylxanthone(1)、4-chloro-1,3,6-trihydroxy-8-methylxanthone(2)、6-hydroxymellein(3)、(R)-4ydroxymellein(4)、3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(5)、2,5-dimethyl-7-hydroxychromone(6)、(S)-regiolone(7)和cy-clonerodiol(8)。结论化合物1～8全部为首次从Hansfordia属真菌中分离得到。Objective To investigate the secondary metabolites of marine sponge-associated fungus Hansfordia sp.Methods The compounds were isolated by sillica gel,Sephadex-LH20 and semi-prepative HPLC chromatography and the NMR,MS spectroscopic analysis were employed for the structural elucidation.Results Eight compounds were isolated from the EtOAc extract of mycelia of Hansfordia sp.and identified as 1,3,6-trihydroxy-8-methylxanthone（1）,4-chloro-1,3,6-triroxy-8-methylxanthone（2）,6-hydroxymellein（3）,4-hydroxymellein（4）,3-（2-hydroxypropyl）-8-hydroxy-3,4-dihydroisocoumarin（5）,2,5-dimethyl-7-hydroxychromone（6）,regiolone（7）and cyclonerodiol（8）.Conclusion All the compounds are isolated for the first time from genus Hansfordia.

关 键 词：海绵共附生真菌 Hansfordia SP 次级代谢产物 化学成分 结构鉴定 

分 类 号：R282.77[医药卫生—中药学]