基于正交设计的毛细管电泳法分离尼卡地平等5种手性药物对映体  

Enantiomeric separation of five basic drugs by orthogonal high performance capillary electrophoresis

作　　者：付小帅[1] 郭雯雯[1] 姜珍[1] 崔艳[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2013年第5期351-355,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273476);辽宁省教育厅科学研究一般项目(L2012353)

摘　　要：目的建立高效毛细管电泳法分离尼卡地平、氧氟沙星、卡维地洛、佐匹克隆和普萘洛尔5种碱性药物对映体。方法采用正交试验设计法,以羧甲基-β-环糊精(carboxymethyl-β-cyclodextrin,CM-β-CD)为手性选择剂,使用未涂层熔融石英毛细管柱,以NaH2PO4缓冲溶液为背景电解质,运行电压为20 kV。结果在最佳条件下,尼卡地平、氧氟沙星、卡维地洛、佐匹克隆和普萘洛尔的对映体分离度分别为3.8、9.8、6.2、5.7和5.0。结论 CM-β-CD对尼卡地平等5种手性药物有较高的对映体选择性。该方法简便、高效、灵敏、重现性好。Objective To establish a high performance capillary electrophoresis method for the chiral separation of nicardipine,ofloxacin,carvedilol,zopiclone and propranolol.Methods Orthogonal experiment design was used to optimize conditions and the separations of the enantiomers were performed in an untreated fused-silica capillary tube with carboxymethyl-β-cyclodextrin（CM-β-CD）in NaH2PO4 buffer as the chiral selector with a high voltage of 20 kV.Results Under the optimal conditions,the resolution of nicardipine,ofloxacin,carvedilol,zopiclone and propranolol were 3.8,9.8,6.2,5.7 and 5.0,respectively.Conclusions CM-β-CD is highly enantioselective for nicardipine,ofloxacin,carvedilol,zopiclone and propranolol.The method is simple,effective and sensitive with an improved reproducibility.

关 键 词：毛细管电泳 正交试验 对映体分离 羧甲基-β-环糊精 尼卡地平 氧氟沙星 卡维地洛 佐匹克隆 普萘洛尔 

分 类 号：R917[医药卫生—药物分析学]