羧甲基-β-环糊精为手性选择剂拆分托吡卡胺等4种手性药物对映体  

Enatioseparation of four drugs by capillary electrophoresis employing carboxymethyl-β-cyclodextrin as a chiral selector

作　　者：刘静[1,2] 袁晓薇[1] 马郑 董斌 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]大连市食品药品检验所,辽宁大连116021

出　　处：《沈阳药科大学学报》2013年第5期374-378,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273476)

摘　　要：目的以羧甲基-β-环糊精(CM-β-CD)为手性选择剂,建立毛细管区带电泳法分离托吡卡胺、文拉法辛、美托洛尔和瑞格列奈4种药物对映体。方法采用未涂壁熔融石英毛细管柱,磷酸盐缓冲液作为背景电解质溶液,分离电压为20 kV。考察了缓冲溶液的pH值、环糊精质量浓度、缓冲盐浓度对对映体分离的影响。结果在优化的分离条件下,4种手性药物均能达到完全分离。结论CM-β-CD适用于上述4种药物的对映体分离。Objective To establish a capillary electrophoresis method for enantiomeric separation of tropicamide,venlafaxine,metoprolol and repaglinide with carboxymethyl-β-cyclodextrin（CM-β-CD） as a chiral selector.Methods The separations of the enantiomers were performed in an untreated fused-silica capillary tube in NaH 2 PO 4 buffer with a voltage of 20 kV.The effects of pH value,chiral selector concentration and concentration of buffer on the enantiomeric separation of the selected drugs were investigated and optimized.Results Under the optimal separation condition,the enantiomers of all drugs examined were completely baseline separated.Conclusions CM-β-CD is highly enantioselective for tropicamide,venlafaxine,metoprolol and repaglinide.

关 键 词：毛细管电泳法 羧甲基-β-环糊精 托吡卡胺 文拉法辛 美托洛尔 瑞格列奈 对映体分离 

分 类 号：R917[医药卫生—药物分析学]