The Ability of Nile Tilapia to Regulate Protein and Energy Intake Evaluated by Carbon Relative Enrichment （δ^13C）  

作　　者：André Moreira Bordinhon Luiz Edivaldo Pezzato Carlos Ducatti Juliana Céia Denadai Margarida Maria Barros 

机构地区：[1]Agriculture and Environment Institute, Universidade Federal do Amazonas, Humaittá, Amazonas 69800-000, Brazil [2]Aquatic Organisms Nutrition Laboratory (Aquanutri), College of Veterinary Medicine and Animal Science, Sǎo Paulo StateUniversity, Botucatu, Sǎo Paulo 18610-307, Brasil [3]Environmental Stable lsotopes Center, Biosciences Institute, Sao Paulo State University, Botucatu, Sao Paulo 18610-307, Brasil

出　　处：《Journal of Agricultural Science and Technology(B)》2013年第1期45-55,共11页农业科学与技术（B）

摘　　要：The aim of this study was to assess the ability of Nile tilapia to balance its own diet, when two ingredient mixes were offered, using carbon stable isotopes. In order to accomplish that, 225 Nile tilapia juveniles （average initial weight 5.0 g ± 0.5 g） were distributed in five tanks, each containing a group of 45 fish. One group of fish were fed exclusively with a high protein mix （HPM; δ^13C = -22.62‰）, the second one fed only with a low protein mix （LPM; δ^13C = -14.34‰）. The other groups had free access to both mixes （free choice system）. The fish from all tanks were fed four times a day. Muscle, liver and blood samples were collected at each five days （from 2 fish/tank/collection） for 86 days, except for the fish fed with LPM （fed for a 120 days period）. The samples were analyzed in a mass spectrometer and proportions of the mixes consumed were estimated through its carbon isotope enrichment （δ^13C）. Energy intake slightly decreased after the 50th day and protein consumption increased after the same period. However, consumption did not present a clear pattern in relation to the individual weight, i.e., protein consumption patterns are mainly related to the age of the individuals and it is not clear if it is also correlated to their weight. Additionally, this technique allowed the observation of differences regarding consumption among the individuals from the experimental group.

关 键 词：Protein intake energy intake diet self-selection carbon stable isotopes Nile tilapia. 

分 类 号：Q959.837[生物学—动物学] S965.125[农业科学—水产养殖]