Carboxylesterase and Glutathione-S-Transferase （GST＇s） Induced Resistance to Bacillus thuringiensis Toxin CrylAb in Rice Leaf Folder, Cnaphalocrocis medinalis （Guenee） Populations  

作　　者：Veegala Ramesh Babu Vemuri Shashi Bhushan Chintalapati Padmavathy: Muthugonder Mohan SenaMahendran. Balachandran Bellamkonda Ramesh 

机构地区：[1]AINP on Pesticide Residues, Acharya N.G. Ranga Agricultural University, Andhra Pradesh, Rajendranagar, Hyderabad 500030,India [2]Division of Crop Protection, Directorate of Rice Research, Rajendranagar, Andhra Pradesh, Hyderabad 500030, India [3]Division of Crop Improvement, Directorate of Rice Research, Rajendranagar, Andhra Pradesh, Hyderabad 500030, India

出　　处：《Journal of Agricultural Science and Technology(A)》2013年第1期53-59,共7页农业科学与技术（A）

摘　　要：The rice leaf folder （RLF）, Cnaphalocrocis medinalis （Guenee） （lnsecta： Lepidoptera： Pyralidae）, is an important pest, widely distributed in many rice growing areas of Asia. The over-use of broad-spectrum chemical insecticides has been cited as a major cause of outbreaks of C. medinalis as excessive spraying of insecticide disrupts natural biological control insecticides still remain the major control tactics against leaf folder. Carbofuran and fenthion, bendiocarb, acephate, carbosulfan, quinolphos, monocrotophos, phosphamidon and fenvalerate are the common ones used against rice leaf folder. Genetically, modified rice lines expressing B. thuringiensis insecticidal crystal proteins produced are highly tolerant to leidopteran pests. Though economic and environmental benefits of GM crops is well established, the matter of concern is the possibility of target insect pest developing resistance to this B. thuringiensis insecticidal toxins, evident from many laboratory and field experiments against many insect pests. The involvement of GSH S-transferase, carboxylesterase, and microsomal monooxygenase in insecticide resistance has been reported in insecticide-resistant strains of many insect species. Hence, the present study was taken up to monitor for cross resistance between B. thuringiensis cry toxins and synthetic insecticides in larvae of leaf folder as it is mediated by carboxylesterase titre and other enzymes by bioassay for two selected rice leaf folder field populations at the Entomology division of Directorate of Rice Research which showed 2-fold resistance ratio. Qualitative and quantitative changes of carboxylesterase （CarE） and glutathione-s-transferase （GST＇s） were worked out with midguts extracts of the two C. medinalis populations in the presence of a-napthyl acetate and chlorodi-nitro benzene substrates.

关 键 词：Cnaphalocrocis medinalis carboxylesterase and glutathione-s-transferase ISOZYMES B type esterases. 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治] X503.225[农业科学—植物保护]