Effect of Sodium Chloride and Proline on Embryo Formation and Germination through in vitro Micropropagation of Date Palm （Phoenix dactylifera L.） cv. Barhee [11]  

作　　者：Sami A1-Zubaydi Abbas Jassim Hussain Zair 

机构地区：[1]Biology Department, Faculty of Science, University of Duhok, Duhok, Kurdistan Region, Iraq [2]Horticulture Department, College of Agriculture, University of Basrah, Basrah, Iraq [3]Biology Department, College of Education, University of Basrah, Basrah, lraq

出　　处：《Journal of Agricultural Science and Technology(B)》2013年第4期313-320,共8页农业科学与技术（B）

摘　　要：The effect of NaCI and proline on somatic embryos formation and germination of date palm （Phoenix dactylifera L.） cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt （NaCI） （0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively） and proline （25, 50 and 75 mg L1） either individually or in combination. Results showed that the addition of NaC1 （starting at 1%） caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations （0.5% and 2%）. No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline （25 mg LL） caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.

关 键 词：Phoenix dactylifera NaCI PROLINE somatic embryos germination. 

分 类 号：S792.99[农业科学—林木遗传育种] Q945.78[农业科学—林学]