基于单片段代换系的水稻抽穗期QTL定位及上位性分析  

QTL Location and Epistasis Analysis of Rice Heading Date using Single Segment Substitution Lines

作　　者：李广贤 柳絮[2] 徐仅婷[2] 王文英[2] 姚方印[2] 

机构地区：[1]山东省水稻研究所/山东省水稻工程技术研究中心,山东济南250100 [2]山东省农业科学院高新技术研究中心,山东济南250100

出　　处：《山东农业科学》2013年第5期30-34,共5页Shandong Agricultural Sciences

基　　金：国家自然科学基金"抽穗期qHD4-1座位的图位克隆和功能分析"(31171529);山东省自然科学基金(Q2008D10)

摘　　要：抽穗期是水稻重要的农艺性状,它决定水稻品种的地域适应性和季节适应性,是重要的育种目标之一。本研究从两个水稻单片段代换系杂交分离群体中筛选到8个次级单片段代换系和2个双片段聚合系,经重叠群作图分析,鉴定出2个与抽穗期有关的QTL。qHD3位于第3染色体短臂,qHD6位于第6染色体的短臂中部。在长日照条件下,Lemont的qHD3等位基因可促进水稻早抽穗,Tetep的qHD6等位基因则延迟水稻抽穗。另外,上位性分析表明,在长日照条件下,qHD3和qHD6之间存在着显著的上位性互作效应。Heading date, an important agronomic trait in rice （ Oryza sativa L. ）, determines the adapta bility to different areas and seasons of rice varieties and is one of the leading objectives in rice breeding. Eight secondary single segment substitution lines （ S - SSSLs） and 2 double segments pyramid lines （DSPLs） were obtained from the F2 population derived from a cross of W23 - 03 - 08 - 9 - 1 and WO1 - 42 - 42 - 8 - 02 - 2, with the same genetic background of Huajingxian74 （HJX74）. Two QTLs were identified with QTL substitu- tion mapping analysis. Under long - day condition, qHD3, on the short arm of chromosome 3, promoted early heading in SSSLs with Lemont allele, while, qHD6, in the middle of short arm of chromosome 6, delayed heading in SSSLs with Tetep allele. Furthermore, there was significant epistatie interaction effect between qHD3 and qHD6 under long -day condition.

关 键 词：水稻 抽穗期 单片段代换系 上位性互作 

分 类 号：S511.2[农业科学—作物学]