拟南芥转化体筛选中Basta喷洒时期和浓度试验  

Experiment on Basta Spraying Period and Concentration in Transgenic Arabidopsis Screening

作　　者：张庆霞[1,2] 魏海蓉[2] 陈新[2] 徐丽[2] 刘庆忠[2] 

机构地区：[1]陇东学院农林科技学院,甘肃庆阳745000 [2]山东省果树研究所/山东省果树生物技术育种重点实验室,山东泰安271000

出　　处：《山东农业科学》2013年第5期96-99,共4页Shandong Agricultural Sciences

基　　金：农业部公益性行业科研专项"樱桃产业主要障碍因素攻关研究"(200903019);农业部"引进国际先进农业科学技术(948)"项目(2011-240);甘肃省庆阳市科技计划项目"甜樱桃优新接穗和抗性砧木品种的引进及早产丰产优质配套技术研究"(KZ2012-77)

摘　　要：为了更加方便有效地利用Basta来筛选带草铵磷(PPT)抗性Bar基因的转基因拟南芥,对不同时期喷洒不同浓度Basta的效果进行了研究。结果表明:拟南芥转化体刚长出2片真叶时对Basta比较敏感,此时开始喷施效果比较好;PPT含量为13.5%的情况下,稀释2 000～4 000倍喷施效果好,此时既可有效筛选出阳性苗,又不会抑制阳性苗的生长;第一次喷施后6天左右移栽阳性苗较好,此时可以明显区分出阳性苗,且移栽后缓苗快、长势好。In order to efficiently screen transgenic Arabidopsis thaliana containing phosphinothricin-resistant Bar gene different concentrations of Basta were sprayed at different periods.The results showed that spraying Basta on Arabidopsis thaliana seedlings with two real leaves,which were sensitive to Basta,would have a good effect;when the PPT content of Basta was 13.5%,a dilution of 2 000~4 000 times was suitable to screen out the resistant plants from non-resistant ones without growth inhibition;it was suitable to transplant the resistant plants at 6th days after the first spraying,because obvious differences appeared between resistant and non-resistant plants and they grew fast and well after transplanted at this time.

关 键 词：拟南芥 喷洒 筛选 Basta 草铵膦(草丁膦 PPT) 

分 类 号：S482.49[农业科学—农药学]