离子束介导玉米DNA的苜蓿变异株AFLP分析  

AFLP Analysis of Alfalfa Variants Transduced by Maize DNA via Ion Beam

作　　者：姬生栋[1] 魏松浩[1] 杨昭昭 张敏 李志勇 马亚锋[1] 郭丹丹[1] 田存章[1] 张洪超[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]焦作市畜牧局,河南焦作454000

出　　处：《河南农业科学》2013年第4期40-43,共4页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金项目(112300410226);省院合作项目(112106000037)

摘　　要：为了培育高产优质苜蓿新品种,利用N+束介导方法将玉米DNA导入苜蓿种子,在当代获得了多种变异植株。采用AFLP分子标记技术,对株高、茎粗、分蘖数差异显著的5种变异植株(3-8、4-12、4-13、4-25、4-26)的基因组DNA进行多态性分析,结果表明,苜蓿变异植株与对照苜蓿间的AFLP扩增图谱相比,分别检测到16、14、12、20、13条差异带,差异率分别为6.3%、5.1%、4.4%、7.1%、4.7%,变异株与对照苜蓿基因组DNA多态性存在明显差异;变异株4-25中检测到与阳性对照玉米一致的2条DNA目的带,为离子束介导玉米DNA转化苜蓿提供了分子证据。Corn DNA was introduced to dry seeds of alfalfa by N＋ low energy implantation and various variants were obtained. The genomic polymorphisms of 5 variants,namely 3-8,4-12,4-13, 4-25 and 4-26,which had some variations in height,diameter and tillers,were analyzed by AFLP marker technique. Some differences were observed between alfalfa mutant lines and alfalfa control,which showed 16,14,12,20, and 13 AFLP polymorphic bands in 3-8,4-12,4-13,4-25, and 4-26,respectively. The differential rates were 6.3% ,5. 1%,4.4% ,7.1% ,and 4.7% ,respectively. Two alfalfa bands which are the same as maize control were found in variant 4-25.

关 键 词：苜蓿 氮离子束介导 AFLP DNA 

分 类 号：S541.1[农业科学—作物学]