热处理对大鼠骨骼肌内质网应激的影响及对骨骼肌适应性的保护作用  

Effects of heat treatment on the endoplasmic reticulum stress of skeletal muscles of rats and its function in protecting the adaptability of skeletal muscles

作　　者：李焕春[1] 许小刚[2] 肖国强[1] 

机构地区：[1]华南师范大学民族体质与健康研究中心/体育科学学院,广东广州510006 [2]黄淮学院体育系,河南驻马店463000

出　　处：《体育学刊》2013年第3期130-133,共4页Journal of Physical Education

摘　　要：为了研究热处理对大鼠骨骼肌内质网应激影响及对骨骼肌的保护作用,采用递增的热处理为研究手段,将SD大鼠被随机分成安静对照组(C)和热处理组(H),其中热处理组按照热处理完成后的时间再分为热处理即刻组(H1)、热处理24 h组(H2)、热处理48 h组(H3)和热处理6 d组(H4),每组8只。结果显示:热处理后H1、H2、H3组CK、LDH浓度都比C组低,但差异无显著性意义;热处理H2组SOD活性最高,与C组比较呈显著性差异(P<0.05),且H2组MDA浓度显著低于C组(P<0.05),其余各组低于C组,但无显著性差异;热处理后H1、H2、H3组骨骼肌中GRP78的表达量与C组呈显著增加。结果表明:热处理后大鼠骨骼肌产生应激蛋白,发生内质网应激反应,并使大鼠骨骼肌产生了适应性保护,从而避免骨骼肌的损伤。In order to study the effects of heat treatment on the endoplasmic reticulum stress of skeletal muscles of rats and its functions in protecting skeletal muscles,by using incremental heat treatment as their research means,the authors divided SD rats randomly into a calm control group（C） and a heat treatment group（H）,further divided the heat treatment group into an immediate after teat treatment group（H1）,a 24 h after heat treatment group（H2）,a 48 h after heat treatment group（H3） and a 6d after heat treatment group（H4） according to the times after heat treatment was finished;each group contained 8 rats.The results showed the followings： after heat treatment,the CK and LDH concentrations of the rats in groups H1,H2 and H3 were lower than those of the rats in group C,but the differences were not significant;the SOD activity of the rats in heat treatment group H2 was the highest,significantly different from that of the rats in group C（P0.05）,and the MDA concentration of the rats in group H2 was significantly lower than that of the rats in group C（P0.05）,the MDA concentrations of the rats in other groups were lower than that of the rats in group C,but the differences were not significant;after heat treatment,the level of expression of GRP78 of the rats in groups H1,H2 and H3 increased significantly as compared with that of the rats in group C.The results indicated the followings： after heat treatment,the skeletal muscles of the rats produced stress proteins,endoplasmic reticulum stress reaction occurred,which caused the skeletal muscles of the rats to produce adaptive protection,thus preventing skeletal muscle damage.

关 键 词：运动生物化学 热处理 骨骼肌 内质网应激 适应性保护 大鼠 

分 类 号：G804.7[文化科学—运动人体科学]