穴位敷贴对过敏性哮喘豚鼠Th/Th2失衡的免疫调节效应  

Immune regulation of Th/Th2 imbalance in allergic asthma guinea pigs by acupoint application

作　　者：顾文[1] 周春祥[1,2] 燕艳[1] 

机构地区：[1]南京中医药大学,南京210046 [2]南京中医药大学明基教学医院,南京210036

出　　处：《中华中医药杂志》2013年第6期1689-1691,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金："十一五"国家科技支撑计划资助项目(No.2008BAI53B068)~~

摘　　要：目的:观察豚鼠血清中白介素-4(IL-4)、γ-干扰素(IFN-γ)含量以及T-bet和GATA-3 mRNA的表达情况,探讨穴位敷贴对过敏性哮喘的免疫调节机制。方法:将30只雄性豚鼠随机分为正常组、模型组、穴位组、非穴位组、阳性对照组(氨茶碱组),每组6只。以卵清白蛋白致敏激发法建立哮喘模型,采用ELISA法检测豚鼠血清中IL-4、IFN-γ含量,RT-PCR法检测肺组织T-bet和GATA-3 mRNA的表达。结果:穴位组与非穴位组IL-4血清含量、GATA-3 mRNA表达明显低于模型组,IFN-γ含量、T-bet mRNA表达明显高于模型组(P<0.01),非穴位组血清IL-4、IFN-γ含量,T-bet mRNA和GATA-3 mRNA的表达与穴位组相比有显著性差异(P<0.01)。结论:穴位敷贴可以通过调节转录因子的表达,从而改变Th0细胞分化方向,影响特征性细胞因子的分泌,纠正Th1/Th2比例失衡,这是穴位敷贴控制哮喘气道炎症的一种可能的机制,且药物敷贴于穴位处较之非穴位处能够发挥更好的疗效。Objective： To observe guinea pig serum IL-4, IFN-y content and T-bet and GATA-3 mRNA expression, to discuss the immune regulation mechanism of acupoint application to allergic asthma. Methods： Thirty guinea pigs were randomly divided into normal group, model group, acupoint application group, non-acupoint application group and positive control group （aminophylline group）, six guinea pigs in each group. The asthmatic model was established by immunization with intraperitoneally injected and inhaled ovalbumin （OVA）. Enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of IL-4 and IFN-y, and reverse transcription polymerase chain reaction （RT-PCR） assay was used for the detection of mRNA expression of T-bet and GATA-3 mRNA. Results： IL-4 content and GATA-3 expressions in the acupoint group and the non-acupuncture point group were significantly lower than the asthma group; IFN-y content and T-bet expressions were obviously higher than those of the asthma group （P〈0.01）. IL-4 and IFN-7 contents, T-bet and GATA-3 mRNA expressions in the acupoint group had significant differences from the non-acupoint group （P〈 0.01）. Conclusion： Acupoint application can change Th0 cell differentiation direction, influence IL-4/IFN-y secretion, correct Thl/Th2 imbalance through regulation of the transcription factors T-bet/GATA- 3 expression which may be one of possible mechanisms acupoint application control asthmatic airway inflammation. Application in acupoint places can play a better curative effect than in non-acupoint places, which may relate to the physiological effects of special points, and this can be a new starting point for further study on acupoint application effect mechanisms.

关 键 词：穴位敷贴 过敏性哮喘 免疫调节 

分 类 号：R245[医药卫生—针灸推拿学]