生精散对大鼠精子特异性钙通道的影响  

Influence of Shengjingsan on cation channel of sperm 1 in rat model

作　　者：王世平[1] 吕魏峰[1] 孙秀斌[1] 徐光玉[1] 孟庆荣[1] 谢克亮[2] 王新生[3] 

机构地区：[1]莱芜市人民医院,莱芜271100 [2]天津医科大学总医院,天津300052 [3]青岛大学医学院附属医院,青岛266071

出　　处：《中华中医药杂志》2013年第6期1692-1695,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学青年基金(No.81101409)~~

摘　　要：目的:研究生精散对雷公藤多甙所制备弱精子症模型大鼠精子特异性钙通道(CatSper1)的影响。方法:60只雄性大鼠随机分为正常对照组、雷公藤多甙组、0.9%氯化钠溶液组及生精散小、中、大剂量组,每组10只。正常对照组每日灌胃0.9%氯化钠溶液1mL/d,其余各组雷公藤多甙20mg.kg-1.d-1,21d后0.9%氯化钠溶液组每日灌胃0.9%氯化钠溶液1mL/d,生精散小(0.01g.kg-1.d-1)、中(0.02g.kg-1.d-1)、大剂量(0.03g.kg-1.d-1)组分别按体质量给予灌服生精散,连续14d,进行精液常规分析,用RT-PCR检测大鼠精子CatSper1的表达。结果:与0.9%氯化钠溶液组比较,生精散各组精子密度、a+b级精子百分率、精子存活率均显著提高(P<0.01),生精散小剂量组CatSper1无明显变化,中、大剂量组CatSper1显著提高(P<0.01)。结论:雷公藤多甙可成功制备弱精子症大鼠模型,中剂量、大剂量生精散可以通过提高CatSper1基因含量,提高精子密度、a+b级精子百分率及精子存活率,从而达到治疗弱精子症的目的。Objective： To study the effect of Shengjingsan on CatSperl in rat model of asthenozoospermia induced by tripterygium wilfordii. Methods： Sixty male rats were randomly divided into control group, tripterygium wilfordii group, NS group, and small-, middle-, large-dose Shengjingsan group, 10 in each group. The rats of control group were given normal saline 1mL·kg-1·d-1, while other groups were given tripterygium wilfordii 20mg·kg-1·d-1, by gavage all for 21 days. Then the NS group was given normal saline 1mL·kg-1·d-1 and the small-, middle-, large-dose Shengjingsan groups were respectively given Shengjingsan （0.01, 0.02, 0.03g·kg-1·d-1） for 20 days. We measured the semen parameters by CASA and CatSperl by RT-PCR in the sperm of rats. Results： The sperm density, percentage of grade a＋b and Sperm viability were remarkably higher in small-, middle-, large- dose Shengjingsan groups than in NS group （P〈0.01）. Compared with NS group, there were no differences in the expressions of CatSperl in small- dose Shengjingsan group, but there were significant differences in middle-, large-dose Shengjingsan groups （P〈0.01）. Conclusion： Tripterygium wilfordii can made asthenozoospermia rats model successfully. Middle-and large- dose Shengjingan can increase the above sperm parameters through increasing the expression of CatSperl, and contribute to the treatment of asthenozoospermia.

关 键 词：精子特异性钙通道 生精散 雷公藤多甙 逆转录聚合酶链式反应 

分 类 号：R285.5[医药卫生—中药学]