鲤鱼汤对阿霉素肾病大鼠肾间充质转分化的影响  

Influence of Cyprinus Carpio Decoction on epithelial-mesenchymal transition of adriamycin nephropathy rats

作　　者：张宇[1,2] 齐玉梅[2] 沈卫[3] 郭云良[3] 孟庆磊[4] 高英[1,2] 陈亚军[2] 宁华英[3] 

机构地区：[1]天津医科大学,天津300170 [2]天津市第三中心医院,天津300170 [3]青岛大学医学院中西医结合中心,青岛266000 [4]山东省淡水水产研究所,济南250117

出　　处：《中华中医药杂志》2013年第6期1845-1848,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072754)~~

摘　　要：目的:研究鲤鱼汤降低肾病综合征大鼠尿蛋白,防止肾间充质转分化(EMT)的作用机制。方法:Wistar雄性大鼠50只,随机分为正常组(N组)、肾病组(S组)、鲤鱼汤低剂量组(CL组)、鲤鱼汤高剂量组(CH组)、厄贝沙坦组(IR组)。一次性尾静脉注射阿霉素6.2mg/kg造模,造模后灌胃相应药物干预。免疫组化法观察肾小管间质整合素链激酶(ILK)、磷酸化糖原合成酶激酶-3β(P-GSK-3β)及α-平滑肌蛋白(α-SAM)表达、β-连环蛋白(β-catenin)胞核阳性率。结果:CL组、CH组、IR组肾小管间质ILK、P-GSK-3β、α-SAM表达强度及β-catenin胞核阳性率较S组降低(P<0.05),ILK及α-SMA表达与肾组织形态变化密切相关。结论:鲤鱼汤可降低阿霉素肾病大鼠尿蛋白,防止EMT。Objective： To investigate the influence of Cyprinus Carpio Decoction on reducting and preventing epithelial- mesenchymal transition. Methods： 50 male Wistar rats were randomly divided into control group （N）, adriamycin nephropathy group （S）, low cyprinus group （CL）, high cyprinus group （CH） and irbesartan group （IR）. Adriamycin nephropathy rat model was established by injecting single tail intravenous 6.2mg/kg. Then begain intervention by gavage after mode,ling. The expression level of integrin-linked kinase （ILK）, p-glycogen synthase kinase 313 （P-GSK-3β）, nuclear β-catenin and smooth muscle a-actin （u-SMA） were examined by immunohistochemistry staining. Results： Compared with S group, the expression of integrin-linked kinase （ILK）, P-GSK-3β, nuclear β-catenin, a-SMA in renal tubular epithelial cells and interstitial significantly dereased by treatment with cyprinus carpio decoction and irb-esartan （P〈0.05）. The expression of ILK and a-SMA were closely related to renal histological changes. Conclusion： Cyprinus carpio decoction could reduce the level of urinary protein in adriamycin uephropathy rats and prevent epitheliai-mesenchymal transition.

关 键 词：阿霉素肾病 鲤鱼汤 整合素链激酶 Β-连环蛋白 肾间充质转分化 

分 类 号：R285.5[医药卫生—中药学]