化浊解毒方药血清对肝星状细胞Ⅰ型胶原与α-平滑肌肌动蛋白的影响  

Affect on collagen Ⅰ and α-smooth muscle actin in hepatic stellate cell by Huazhuo Jiedu Herbs

作　　者：邸淑珍[1,2] 卜宝鹏[1,2] 高绍芳[1,2] 

机构地区：[1]河北医科大学,石家庄050200 [2]河北省中医院,石家庄050011

出　　处：《中华中医药杂志》2013年第6期1867-1869,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河北省科技厅科技支撑项目(No.10276105D-85)~~

摘　　要：目的:本研究观察化浊解毒中药对肝星状细胞(HSC)I型胶原与α-SMA的影响,以探求其治疗肝纤维化的细胞分子学机制。方法:采用RT-PCR检测化浊解毒方药血清对肝星状细胞细胞I型胶原和α-SMA表达的影响。结果:与正常血清组比较,各组均能抑制HSC I型胶原的分泌(P<0.05),其中中药血清大剂量组与干扰素组、中药中剂量组、中药小剂量组比较有显著性差异(P<0.05),而干扰素组、中药中剂量组、中药小剂量组之间比较无显著性差异;与正常血清组比较,各组均能抑制HSC-T6分泌α-SMA的表达(P<0.05),其中中药大、中剂量组更为明显(P<0.05),中药小剂量组与干扰素组之间比较无显著性差异。结论:化浊解毒中药能够减少I型胶原、α-SMA的表达,并且有一定的量效关系。从而推断化浊解毒中药可以抑制HSC增殖及活化,抑制肝纤维化的发生和发展。Objective： To explore the cellular and molecular of Huazhuo Jiedu Herbs on collagen I and α-SMA of liver fibrosis. Methods： By using RT-PCR to detect the expression of collagen I and α-SMA by Huazhno Jiedu Herbs. Results： With the blank control group, each group could inhibit hepatic stellate cell type I collagen, α-SMA expression （P〈0.05）. The pairwise comparison results indicate that, compared with normal serum, the groups could inhibit the amount of the HSC secretion collagen I, which Huazhuo Jiedu Herbs serum high-dose group and interferon group, middle dose group, low-dose group more significantly （P〈0.05）, no significant difference between the interferon group, dose group in the traditional Chinese medicine, Chinese medicine, low-dose group; hepatic stellate cells results showed that α-SMA, and normal serum group compared to control treatment group could inhibit the expression of HSC-T6 secretion of α-SMA （P〈0.05）, which Huazhuo Jiedu Herbs, the middle dose group was more significant （P〈0.05） detoxification Huazhuo small dose group and interferon no significant difference between the groups. Conclusion： Huazhuo Jiedu Herbs can reduce the expression of collagen I and α-SMA, and the dose-effect relationship. Thus infer Huazhuo Jiedu Herbs can inhibit HSC proliferation and activation, inhibition of the occurrence and development of liver fbrosis.

关 键 词：肝星状细胞 化浊解毒中药 Ⅰ型胶原 Α-平滑肌肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]