亚硫酸氢钠穿心莲内脂对小鼠肾毒性及其蛋白表达分析  

Renal toxicity of andrographolide sodium bisulfite in mice and analysis of protein expression

作　　者：周彦铨[1] 陆红[2] 吴峰[3] 

机构地区：[1]浙江省农业科学院卫生所,杭州310021 [2]浙江中医药大学药学院,杭州310053 [3]浙江大学附属医院第一医院,杭州310009

出　　处：《中华中医药杂志》2013年第6期1890-1892,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:观察亚硫酸氢钠穿心莲内脂(ASB)的肾毒性机制。方法:20只雄性ICR小鼠随机分为正常对照组和ASB组(1 000mg/kg),每组10只。小鼠尾静脉注射ASB,每天1次,连续7d,正常对照组用等体积0.9%氯化钠溶液同条件予以注射。进行血常规、血液生化指标及肾病理组织学检查。另取一肾提取蛋白质进行双向凝胶电泳分离找出差异蛋白点。结果:ASB组,外周血白细胞从(10.50±2.00)×109/L升高至(13.70±3.40)×109/L,血小板从(666.05±122.09)×l09/L降低至(451.31±207.22)×109/L,淋巴细胞比例从(0.83±0.03)降低至(0.68±0.11);血清尿素氮、肌酐异常升高(P<0.05);其中8只小鼠出现肾脏病变,多为肾小管间质炎症细胞浸润(间质性肾炎)。肾组织蛋白经双向电泳后,ASB组与正常对照组比较有8个差异蛋白点:2个表达增强,2个表达降低,新出现2个,消失1个,移位1个,有显著性差异变化(P<0.05)。结论:在通过肾组织蛋白表达分析后,ASB所致小鼠急性肾损伤且组织有明显不同蛋白差异点表达,这些差异蛋白质可能参与了ASB肾毒性损伤的过程。Objective： To study the mechanism of nephrotoxicity by andrographolide sodium bisulfite （ASB）. Methods： 20 male ICR mice were randomly divided into normal control group and ASB1 000mg/kg group. Mice in ASB group were given ASB 1 000mg/kg by tail vein, once daily for 7d. The normal control group was given an equal volume of sailine at the same conditions. Examining blood routine and blood biochemical indexes detection; taking a kidney for histopathological examination. Another kidney were homogenated and renal proteins were extracted, differential proteins were expressed by the two-dimensional （2-D） electrophoresis proteomic. Results： In ASB 1 000mg/kg, white blood cells elevated from（10.50±2.00）x109/L to （13.70±3.40）x109/L. Platelets reduced from（666.05±122.09）x109/L to （451.31±207.22）x109/L, the proportion of lymphocytes reduced from（0.83±0.03） to （0.68±0.11）; serum BUN, CR were elevated respectively （P〈0.05）; 8/10 mice in this group had renal lesions which mostly were renal tubulointerstitial inflammatory cells infiltration（interstitial nephritis）. 8 protein spots were obtained after two-dimensional electrophoresis： there were 2 spots expression increasing, 2 spots expression decreasing, 2 new protein spots emerging, 1 spot disappearing, 1 spot shift in treatment group. There were some significant changes （P〈0.05）. Conclusion： Different spots were expressed between the two groups, and different protein might be participated in the course of injury kidney by ASB.

关 键 词：亚硫酸氢钠穿心莲内脂 肾毒性 机制 双向凝胶电泳 

分 类 号：R285[医药卫生—中药学]