相关miRNAs对LS174T细胞中MRPs的影响  

Effect of relative miRNAs on MRPs in LS174T cells

作　　者：应梦佳[1] 毕惠嫦[1] 胡冰芳[1] 周许年[1] 金晶[1] 钟国平[1] 黄民[1] 

机构地区：[1]中山大学药学院药物代谢与药动学实验室,广东广州510006

出　　处：《中国药理学通报》2013年第6期778-782,共5页Chinese Pharmacological Bulletin

基　　金：国家"十二五"科技重大专项(No 2012ZX09506001-004);广东省重点实验室项目(No 2011A060901014)

摘　　要：目的探讨相关miRNAs对多药耐药蛋白MRPs转录的影响。方法经脂质体瞬时转染,将pCMX-miR-328,pC-MX-miR-206及pCMX-miR-613表达质粒转入LS174T细胞,24h后采用荧光定量RT-PCR检测相关MRPs的mRNA变化。结果在LS174T细胞内过表达hsa-miR-328,能够降低MRP3的mRNA表达水平,其抑制率为0.32(P<0.05);hsa-mir-206能够降低MRP1的mRNA表达水平,其抑制率为0.35(P<0.05)。hsa-mir-613对LS174T细胞中的相关MRPs表达无影响。结论 hsa-miR-328和hsa-miR-206在LS174T细胞系中可以抑制MRP3和MRP1的转录表达。Aim To investigate the transcriptional effect of hsa-miR-328, hsa-miR-206 and hsa-miR-613 on muhidrug resistance-associated protein （MRP1MRP3, and MRP5）. Methods LS174T cells were transient transfected with pCMX-miR-328, miR-206, and miR - 61 3 expression vectors. Twenty - four hours af-ter the treatment, mRNA level of MRP1, MRP3 and MRP5 was detected by real-time PCR. Results The mRNA expression level of MRP3 was significantly in- hibited by hsa-miR-328 in LS174T cells. The MRP1 mRNA expression was significantly inhibited by hsa- miR-206 in LS174Tcells. However, the mRNA expres-sion level of MRP1, MRP3, and MRP5 was not influ- enced by has-mir-613. Conclusion Specific MRPs can be down-regulated after transfected with pCMX- miR-328 or 206 in LS174T cells.

关 键 词：miRNA MRP 基因调控 多药耐药 mRNA 转染 

分 类 号：R341[医药卫生—基础医学] R342.22