制备微藻油脂的转基因小球藻生长的影响因素分析  

Analysis on Factors Affecting the Growing of Transgenic Chlorella for Preparing Microalgae Oils

作　　者：李瑶[1] 王雷[2] 付玉杰[1] 

机构地区：[1]东北林业大学林业生物制剂教育部工程研究中心/森林植物生态学教育部重点实验室,哈尔滨150040 [2]黑龙江省科学院高技术研究院,哈尔滨150000

出　　处：《湖北农业科学》2013年第8期1905-1907,共3页Hubei Agricultural Sciences

摘　　要：利用从加拿大引进的转基因小球藻(Chlorella vulgaris)制备微藻油脂,探讨影响小球藻生长的条件,如培养基pH、培养温度等,以期获得脂肪酸组成以C16和C18为主的藻株及培养条件。结果表明,将转基因小球藻接种在氮磷质量比为4∶3、pH 8.5的GM-SC培养基中,在28℃进行培养,最终可得到生产状况较好、生长速度较快的小球藻。Transgenic chlorella（Chlorella vulgaris） imported from Canada was used to prepare microalgae oil.The conditions for the growth of chlorella such as medium pH and culturing temperature were discussed to get chlorella strain and cultural conditions that could produce oil mainly composed with C16 and C18.The results showed that when the chlorella was inoculated on GM-SC medium with mass ration of N to P at 4∶3,pH 8.5,and cultured at 28 ℃,the growth condition of chlorella was well and the growth speed was fast.

关 键 词：小球藻(Chlorella vulgaris) 微藻油脂 脂肪酸 转基因 

分 类 号：TK6[动力工程及工程热物理—生物能]