乙型肝炎病毒表面抗体快速测定方法应用评价  

Evaluation of Rapid Determination Method for Hepatitis B Virus Surface Antibody

作　　者：范黎[1] 王保宁[2] 林红[1] 杨康林[1] 魏承斌[1] 王俊 

机构地区：[1]乐山市疾病预防控制中心,四川乐山614000 [2]四川大学华西基础医学与法医学院 [3]四川迈克生物科技股份有限公司

出　　处：《预防医学情报杂志》2013年第5期356-358,共3页Journal of Preventive Medicine Information

基　　金：四川省卫生厅和乐山市科技局立项课题;并获乐山市科技进步一等奖

摘　　要：目的对新开发的一种快速、敏感的乙型肝炎病毒表面抗体(乙肝疫苗保护性抗体)测定方法进行应用评价。方法与酶标法试剂盒同时对1 042份血清样本,62份血浆样本、92份全血样本进行临床比对测定。结果对1 042例临床血清样本、62份血浆样本和92份全血样本进行临床比对实验,与酶标法比对试剂符合率分别为96.2%、96.8%和95.7%。结论该方法能测定人的全血、血清、血浆样本中的乙型肝炎病毒表面抗体。本试纸条10 min内即可完成检测,具有简便快速、无需特殊仪器设备等优点,对测定人群中乙型肝炎病毒表面抗体或乙肝疫苗接种后人群中乙肝疫苗保护性抗体产生效果评价具有应用价值。Objective To develop a rapid and sensitive method to determine hepatitis B surface antibody （hepati-tis B vaccine protective antibodies）, and to evaluate the application effect. Methods Clinical comparison test were conducted with the enzyme linked immunosorbent assay kit. Results Totally 1042 serum samples, 62 plasma samples and 92 clinical whole blood samples were compared. The coincidence rates were 96. 2%, 96.8% and 95.7%, respectively. Conclusion The method can determine human＇s whole blood, serum, plas- ma samples of hepatitis B virus surface antibody, with characteristics of easy and fast, thus hold a potential ap- plication value.

关 键 词：乙型肝炎病毒表面抗体 快速测定试纸 

分 类 号：R446.11[医药卫生—诊断学]