上海奉贤区公共场所嗜肺军团菌污染状况调查  

Legionella Pneumophila Pollution in Public Places of Fengxian District,Shanghai City

作　　者：沈莉[1] 谢晓红[1] 韩华忠 陶力新 袁建华[1] 王仁刚[1] 

机构地区：[1]上海市奉贤区疾病预防控制中心,上海201400

出　　处：《预防医学情报杂志》2013年第5期407-410,共4页Journal of Preventive Medicine Information

摘　　要：目的应用PCR技术对奉贤地区部分公共场所空调冷却塔水及其他环境(淋浴设施等)进行军团菌污染状况调查。方法于2010-05和2010-10,分别采集中央空调冷却塔水样品及环境样品用聚合酶链(PCR)对其进行嗜肺军团菌检测,同时用常规法进行分型鉴定。结果采集的125份样品中,PCR法检出嗜肺军团菌45份,阳性检出率为36%,水样检出率远高于环境(分别为67.19%和3.28%)。在检出的59株军团菌中共鉴定出6种不同菌型,其中LP1为重点菌型,占50.85%。且在同一样品中检出不同菌型有2株以上的占35.29%。结论奉贤地区中央空调冷却塔及环境中存在军团菌污染。Objective With technique of polymerase chain （PCR） to investigate legioneUa contamination in pub-lic places by testing samples from air-conditioning cooling tower water and shower facilities in Fengxian dis-trict, Shanghai. Methods Samples from central air -conditioning cooling tower and other environmental facili-ties were tested with PCR technique in May and October, respectively; and classification identification was car-tied out with routine techniques. Results Among the 125 samples collected, 45 were identified legionella pneu- monia, with a positive rate of 36% ; higher rate （67.19%） appeared in water than in other environment （3.28%）. There were 6 different bacteria types identified in 59 strains of legionella, and LP1 was the key bacteria type, accounting for 50. 85%. The portion of no less than 2 types in one single sample was 35.29%. Conclusion Legionella contamination exists in air-conditioning cooling tower water and other environments in Fengxian＇district; necessary attention needs to be paid for sake of preventing legionella outbreak.

关 键 词：军团菌 冷却塔 PCR 

分 类 号：R126.4[医药卫生—环境卫生学]