响应面优化混菌固态发酵豆粕制备多肽饲料的发酵条件  

Optimum fermentation conditions of preparing soybean opypeptide from fermented soybean meal by mixed strains with response surface methodology

作　　者：王哲奇[1] 齐景伟[1] 安晓萍[1] 陈大勇 仝宝生 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 [2]内蒙古斯隆生物技术有限责任公司,内蒙古呼和浩特011517

出　　处：《饲料工业》2013年第9期53-57,共5页Feed Industry

基　　金："十二五"国家科技支撑计划项目[2011BAD18B02]

摘　　要：利用响应面分析法对枯草芽孢杆菌和米曲霉混合固态发酵豆粕发酵条件进行优化,提高多肽含量。运用minitab 16软件,首先采用Plackett-Burman方法分析6种因素对产生多肽量的影响,确定装料量、发酵时间、发酵温度为影响多肽产量的重要因素。然后采用响应面法(response surface methodology,简称RSM)对这三个因素进行优化。得到最优条件:装料量10 g;发酵时间96 h;发酵温度34℃。此时大豆多肽含量可达21.32%,与响应面优化得到的理论值仅相差0.65%。Response surface methodology was applied to optimize the fermentation of bacillus subtilis and aspergillus oryzae for the content of soybean peptid. In the first step, Plackett-Burman was used to evaluate the influence of six related factors by minitab 16. The results showed that the effects of fermentation volume, fermentation lasting time and fermentation temperature on the activities of soybean peptid were significant among the six factors. In the follow step, the optimal conditions of the three factors were assessed by the RSM. The optimal conditions of the three factors were as follows： fermentation volume 10 g, fermentation lasting time 96 h and fermentation temperature 34℃. Under these conditions, the content of soybean peptid was 21.32% after optimization of which only 0.65% difference from the forecast by RSM.

关 键 词：大豆多肽 响应面 豆粕 枯草芽孢杆菌 米曲霉 

分 类 号：S816.3[农业科学—饲料科学]