羊传染性脓疱病毒吉林分离株F1L基因的克隆与同源性分析  

Cloning and Homology Analysis of F1L Gene of Orf virus Isolated from Jilin Strain

作　　者：邵洪泽[1,2] 程荣华[2] 石春军 呼延含蓉 黄海楠[2] 许尧[2] 毛文智[2] 胡桂学[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]吉林省畜牧兽医科学研究院,长春130062 [3]前郭县动物疫病预防控制中心,吉林松原131100

出　　处：《吉林大学学报（理学版）》2013年第3期518-522,共5页Journal of Jilin University:Science Edition

基　　金：吉林省科技发展计划项目(批准号:20110235)

摘　　要：通过参考GenBank中羊传染性脓疱病毒的F1L基因序列设计引物,应用PCR技术的特异性扩增了羊传染性脓疱病毒JLSY04株的F1L基因片段,测序得到了该病毒F1L基因序列,并与几个参考毒株序列进行比对.实验结果表明,羊传染性脓疱病毒JLSY04株与OV/Torino株同源性最低,为96.0%,与Jilin株同源性最高,为99.4%.即该羊传染性脓疱病毒JLSY04株F1L基因与其他参考毒株间的差异较小,可作为基因工程疫苗的目的基因.According to the DNA sequence of the F1L gene which was stored in GenBank.the authors designed one pair of primers to amplify F1L gene of JLSY04 by PCR.The amplified positive production was sequenced and the sequence of it was compared with the sequences of several reference strains domestic-isolated.The results show that JLSY04 stain and OV/Torino strain show the lowest homology of 96.0%with respect to F1L gene,and the highest homology of Jilin strain was up to 99.4%.There was little difference of F1L gene between JLSY04 strain and each of other reference strains isolated from different species and different areas in China.The analysis result of homology suggests that the F1L gene can be used as the target genes of the genetically engineered vaccine.

关 键 词：羊传染性脓疱病毒 F1L基因 克隆 同源性分析 

分 类 号：Q93[生物学—微生物学]