双歧杆菌介导的福氏志贺菌Bb-ipaB疫苗的构建  

Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri

作　　者：王国富[1] 薛士鹏[1] 白丽[1] 吴利先[1] 

机构地区：[1]大理学院基础医学院,云南大理671000

出　　处：《大理学院学报（综合版）》2013年第3期13-15,共3页Journal of Dali University

基　　金：云南省教育厅基金重点项目基金资助项目(09Z0078)

摘　　要：目的:构建福氏志贺菌重组双歧杆菌Bb-ipaB疫苗。方法:以福氏志贺菌DNA为模板扩增福氏志贺菌ipaB基因,双酶切后克隆到pGEX-1λT质粒上,构建重组质粒pGEX-ipaB,电穿孔法将该质粒转化入Bb,构建福氏志贺菌重组Bb-ipaB疫苗。结果:成功扩增出分子量约为1 711 bp的ipaB基因,双酶切证实基因成功插入pGEX-1λT中,并成功转化入双歧杆菌,构建rBb-ipaB疫苗。结论:成功构建福氏志贺菌重组rBb-ipaB疫苗,为该疫苗的进一步研究奠定了基础。Objective：To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods：ipaB gene was amplified by PCR and cloned into pGEX-1 λ T plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum （Bb） to construct rBb-ipaB vaccine. Results： A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1 λ. T by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion：The rBb- ipaB vaccine of Shigellaflexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.

关 键 词：福氏志贺菌 双歧杆菌 重组Bb-ipaB疫苗 构建 

分 类 号：R378.2[医药卫生—病原生物学]