狼疮性肾炎患者外周血CD4+CD25^(high)调节性T细胞检测临床意义  

Frequency and Significance of CD4 + CD25^(high) Regulatory T Cells in the Peripheral Blood of Lupus Nephri tis Patients

作　　者：马纪林[1] 蔡龙[1] 施华萍[1] 周红娟[1] 张风卫[1] 于健宁[1] 陶筱娟[1] 

机构地区：[1]杭州市红十字会医院风湿免疫科,杭州310003

出　　处：《浙江中西医结合杂志》2013年第5期348-349,367,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金:(No.81274161;81001307);浙江省自然科学基金(No.Y2090918);浙江省医药卫生科学研究基金计划项目(No.2012RCA046);杭州市科技发展计划项目(No.20080333Q28)

摘　　要：目的:检测狼疮性肾炎患者外周血CD4+CD25high调节性T细胞的比例,探讨其临床意义。方法:采用流式细胞仪检测35例狼疮性肾炎患者及11名健康对照者的外周血CD4+CD25high调节性T细胞的比例,并分析其与肾脏病理类型和疾病活动的相关性。结果:狼疮性肾炎患者外周血CD4+CD25high调节性T细胞的比例与健康对照组相比,差异无统计学意义(P>0.05),并与疾病活动性无相关性。但III型和IV型狼疮性肾炎患者,CD4+CD25high调节性T细胞的比例较II型和V型患者明显降低(P<0.05)。结论:检测外周血CD4+CD25high调节性T细胞的比例可以辅助判断狼疮性肾炎的病理类型,并可为治疗狼疮性肾炎提供理论依据。Objective： To investigate the frequency of CD4＋CD25high regulatory T cells in the peripheral blood of lupus nephritis patients with diverse pathology. Methods： Peripheral blood mononuclear cells from 35 lupus nephritis patients based on the 2003 ISN/RPS classification system and 11 healthy controls were counted and stained for CD4 and CD25. The relation between CD4＋CD25high regulatory T ceils and renal pathology anddisease activity was analyzed. Results： No significant difference in the frequency of CD4＋CD25high regulatory T cells was noted between the lupus nephritis patients and normal controls （P〉0.05）. Although the frequency of this cell populations was not correlated to the disease activity （P〉0.05）, it was significantly decreased in lupus nephritis patients with Class III and IV when compared with patients with Class II and V （P〈0.05）. Conclusion： CD4＋CD25high regulatory T cells in the peripheral blood could be a promissory biomarker for active lupus nephritis patients and this may give rationale to use regulatory T cells as target cells in the treatment of lupus nephritis.

关 键 词：狼疮性肾炎 调节性T细胞 肾脏病理 

分 类 号：R593.242[医药卫生—内科学]