铜(Ⅱ)-曙红B-洛美沙星的共振瑞利散射的研究与应用  

Study on resonance Rayleigh scattering of copper(Ⅱ)-eosin B-lomefloxacin system and its application

作　　者：刘长鹤[1] 李贵荣[1] 吕威风[1] 李秋[1] 赵慧[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2013年第5期932-935,共4页Applied Chemical Industry

摘　　要：在pH 4.0的BR缓冲介质中,洛美沙星能与铜(Ⅱ)形成螯合阳离子,再通过静电引力和疏水作用,与曙红B阴离子形成缔合物,导致共振瑞利散射(RRS)显著增强。在最大RRS峰380 nm处,散射光增强值(ΔI)与洛美沙星的浓度成正比,据此建立了共振光散射法测定洛美沙星,线性范围为8.99×10-8～3.00×10-6mol/L,检出限(3Sb/k)为2.69×10-8mol/L。该法用于检测牛奶中洛美沙星的含量,并取得满意结果。该方法简单、快速、灵敏度高。In pH 4.0 Britton-Robinson （BR） buffer solution, lomefloxacin could react with copper （Ⅱ） to form chelate cations, which further bind with eosin B to form association. This resulted in the obvious enhancement of resonance Rayleigh scatter/ng （RRS）. The maximum RRS wavelength situated at 380 nm. The intensities of RRS at 380 nm are proportional to the concentrations of lomefloxacin in the ranges of 8.99× 10^-8 - 3.00 × 10^-6 tool/L, the detection limit （3Sb/k） is 2.69× 10^-8 mol/L. Based on this, a new method was developed for the determination of FLQS in milk, the proposed method was simple, rapid and sensitive.

关 键 词：洛美沙星 铜(Ⅱ) 曙红B 共振瑞利散射 

分 类 号：TQ203.9[化学工程—有机化工] O657.32[理学—分析化学]