低盐胁迫对黄鳍棘鲷幼鱼存活率、鳃ATP酶和肝脏抗氧化酶的影响  

Effects of Low Salinity Stress on Survival Rate,Gill ATPase and Liver Antioxidant Enzymes in the Young Yellowfin Porgy Acanthopagrus latus

作　　者：朱友芳 肖懿哲 李文辉 洪万树[2] 毛勇[2] 

机构地区：[1]莆田市水产科学研究所,福建莆田351100 [2]厦门大学海洋与地球学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2013年第3期414-420,共7页Journal of Xiamen University：Natural Science

基　　金：国家海洋公益性行业科研专项(201005013);莆田市科技局项目(2011N03)

摘　　要：为了解低盐胁迫对黄鳍棘鲷(Acanthopagrus latus)养殖所产生的影响,采用快速或缓慢2种梯度淡化方法,研究盐度由20淡化至10,5甚至淡水后对黄鳍棘鲷幼鱼的存活率、鳃ATP酶和肝脏抗氧化酶的影响.结果表明:1)除了淡水组的黄鳍棘鲷全部死亡之外,其他低盐组中的成活率为100%;2)在响应不同盐度的低盐胁迫时,不同酶的敏感性有所不同,其中超氧化物歧化酶(SOD)仅对淡水胁迫敏感,Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶对盐度10的低盐胁迫不敏感,过氧化氢酶(CAT)对所有的实验组都敏感,盐度胁迫强度由强到弱依次为淡水、盐度5和盐度10;3)不同的淡化速度影响着Na+/K+-ATP酶和CAT活力恢复到正常水平所需的时间,其中快速淡化所需时间要比缓慢淡化长.本研究结果表明黄鳍棘鲷幼鱼可以较好适应低盐度养殖环境,但淡化速率会对黄鳍棘鲷幼鱼产生一定的应激影响,并且在淡水中应激非常明显.To investigate the effect of the low salinity stress on Acanthopagrus latus farming,a large or small gradient desalination rate was set from 20 to 10,5 or even to fresh water,which was applied to study the impact of low salinity on survival,ATPase in gill and antioxidant enzymes in liver. The results showed that ： 1） All the individuals of the group in fresh water died while survivals of other groups were 100 %;2）The effect of low salinity stress on four kinds of enzymes were different,SOD was not sensitive but to fresh water,Na＋/K＋-ATPase and Caz＋/Mg2＋-ATPase were insensitive to the salinity of 10, CAT was sensitive to all groups. The stress intensity of low salinity on enzymatic activities enhanced with desalination extent to 10,5,or finally to freshwater;3）The recov ery time of Na＋/K＋-ATPase to normal activity was affected by the desalination rate：the faster desalination, the slower recovery. The findings suggested that young A. latus can be adaptable to low salinity, but may produce some stress response under quick desalina tion,apparently to fresh water.

关 键 词：黄鳍棘鲷 低盐胁迫 存活率 ATP酶 抗氧化酶 

分 类 号：S965.3[农业科学—水产养殖]