铅、锌及其复合胁迫对花菖蒲幼苗生长及生理抗性的影响  

Effects of Lead, Zinc and Their Combined Stress on Growth and Physiological Response of IIrris ensata Seedling

作　　者：付佳佳[1] 韩玉林[2] 赵九洲[2] 许敏[2] 

机构地区：[1]南昌工学院,江西南昌330108 [2]江西财经大学艺术学院园林系,江西南昌330032

出　　处：《湿地科学》2013年第2期198-203,共6页Wetland Science

基　　金：国家自然科学基金项目(30771520);江西省研究生创新项目(YC09A086)资助

摘　　要：通过水培方法,研究了铅(Pn)和锌(Zn)及其复合胁迫对花菖蒲(Iris ensata)幼苗生长与生理指标的影响。将花菖蒲栽植于300mL培养瓶中,以1/2Hoagland营养液培养7d,在0.5mmol/L Pb+1mmol/L Pb处理下,花菖蒲幼苗地上部干重与对照组相比差异显著;其他处理与对照处理相比差异不显著;在所有Pb和Zn及其复合胁迫处理下,花菖蒲地下部干重与对照处理相比没有显著差异。除了在1mmol/L Zn处理下,花菖蒲光合色素含量与对照处理相比没有显著差异之外,其他处理的光合色素含量都比对照处理有所减少。高浓度Pb和Pb+Zn的复合胁迫对花菖蒲幼苗地上部的过氧化物酶活性诱导能力很强,在0.5mmol/LPb+1mmol/LZn复合胁迫下,花菖蒲幼苗地上部的过氧化物酶活性最高,比对照处理高81.8%;而其超氧物歧化酶活性在高浓度Pb和Zn处理下受到一定抑制;在0.5mmol/LZn胁迫下,花菖蒲地上部超氧物歧化酶活性最高,是对照处理的1.1倍。除单因子Zn处理外,花菖蒲植株地上部丙二醛的含量与对照处理相比都显著上升。研究结果说明,过氧化物酶在花菖蒲抗单因子Pb和Zn及其复合胁迫中起到了非常重要的作用,花菖蒲对Pb和Zn具有较强的耐受性,可以用于修复Pb和Zn及其复合污染的环境。The effects of lead（Pb）, zinc（Zn） and their combined stress on the growth and physiological parameters of Iris ensata were studied by liquid culture. Results show that the aboveground part dry weights of Iris en？ sata seedling under 0.5 mmol/L Pb and 1 mmol/L Pb treatments increased significantly by 15.2% and 12% compared with the control （CK）. The dry weights of aboveground parts under other treatments were no significant difference with that of the control. However, the underground part dry weights of Iris ensata seedling under all treatments were no significant difference with that of the control. All photosynthetic pigment contents in the leaves decreased significantly compared with the control except 1 mmol/LZn treatment. The peroxidase （POD） activities in leaves of Iris ensata seedling treated with 1 mmol/L Pb and 0.5 mmol/LPb ＋1 mmol/L Zn were promoted, and the POD activity under 0.5 mmol/LPb＋1 mmol/LZn stress was 81.8% higher than that of control. However, the superoxide dismutase（SOD） activities under high concentration of Pb and Zn treatments were inhibited to a certain extent, and the SOD activity in leaves under 0.5 mmol/LZn treatment was the highest and 1.1 times of the control. The malondialdehyde（MDA） contents in leaves of Iris ensata seedling were increased significantly compared with that of control except single Zn treatments. The experiment results indicated that the seedlings of Iris ensata have strong tolerability against Pb and Zn and POD played an important role to against Pb, Zn and their combined stress and the Iris ensata seedling can be used to remediate the Pb and Zn contaminated environment.

关 键 词：花菖蒲 铅 锌 胁迫 生理反应 

分 类 号：Q494[生物学—生理学]