山羊绒毛干中线粒体DNA和核DNA的提取与PCR扩增  

DNA Extraction from Cashmere Goat and Its PCR Amplification

作　　者：耿荣庆[1,2] 周光现[1] 韩旭峰[1] 李伟[1] 李碧波[1] 王小龙[1] 陈玉林[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]盐城师范学院生命科学与技术学院,江苏盐城224051

出　　处：《家畜生态学报》2013年第3期13-15,共3页Journal of Domestic Animal Ecology

基　　金：农业部转基因生物新品种培育科技重大专项(2011ZX08008-002);国家自然科学基金资助项目(31101684);西北农林科技大学拔尖人才支撑计划;江苏省高校"青蓝工程"人才培养项目

摘　　要：本研究以PCR缓冲液、MgCl2溶液和蛋白酶K为裂解提取液,建立了一种从山羊绒毛干中快速提取DNA的方法。结果表明,该方法不仅能从山羊绒毛干中提取出线粒体DNA和核DNA,而且能够有效地进行线粒体基因和核基因组基因的PCR扩增,为拓展羊绒样品在个体识别、遗传资源评价、分子进化和分子标记辅助育种等研究领域中的应用奠定基础。This study established a rapid DNA extraction method from goat cashmere without hair follicle by using the lysis solution including PCR buffer, MgCl2 and proteinase K. The results showed that it could not only extract mitochondrial DNA and nuclear DNA form cashmere shaft at the same time but also complete PCR amplification efficiently. It would provide a foundation for expanding the application of cashmere samples in individual recognition, genetic resources assessment, molecular evolution, molecular marker assisted breeding and other research areas.

关 键 词：山羊绒 线粒体DNA 核DNA DNA提取 PCR扩增 

分 类 号：S811.6[农业科学—畜牧学]