HPLC法测定清瘟败毒颗粒中盐酸小檗碱的含量研究  

Determination of Berberine Hydrochloride in Qingwen Baidu Granule by HPLC

作　　者：郭时金[1,2] 王艳萍[1,2] 周春凤[1,2] 董林[1] 徐倩倩[1,2] 张志美[1,2] 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都安特动物药业有限公司,山东滨州256600

出　　处：《家畜生态学报》2013年第4期37-40,共4页Journal of Domestic Animal Ecology

基　　金：山东省技术创新项目(201210916001)

摘　　要：为了建立"清瘟败毒颗粒"中盐酸小檗碱含量的高效液相色谱检测方法(high-per-formance liquid chromatography,HPLC),采用SB-C18柱(StableBond,4.6×150 mm,5μm),流动相:乙腈-0.05mol/L磷酸二氢钾溶液(50∶50),紫外检测波长为345nm,流速1.0mL/min,柱温30℃。在选择色谱条件下,盐酸小檗碱与辅料、溶剂及清瘟败毒颗粒中的其他成分的峰之间分离良好,在16.0～48.0μg/mL范围内峰面积与盐酸小檗碱含量直线关系(r2=1,n=6),平均回收率为99.09%。该方法简便、灵敏、可重复性好,可用于清瘟败毒颗粒的质量控制。To establish a simple and sensitive HPLC method for determining berberine hydrochloride in Qingwen Baidu granule,HPLC method was used. The HPLC system consisted of Stable Bond C18 column （4.6×150mm,5μm） and a mobile phase of acetonitrile-0.05mol/L potassium dihydrogen phosphate solution （50 ： 50, v ： v） mixture. Berberine hydrochloride was detected at 345 nm, with a flow rate of 1.0 mL/min. The column temperature was maintained at 30 ℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0-48.0μg/mL. The average recovery of berberine Hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable. It could be used to control the quality of Qingwen Baidu granule.

关 键 词：盐酸小檗碱 高效液相色谱法 清瘟败毒颗粒 

分 类 号：S811.6[农业科学—畜牧学]