广东省鼠疫静息期3种耶尔森氏菌鼠疫PCR检测  

PCR assay detection for three types of Yersinias isolated in plague static phase in Guangdong

作　　者：黄济英 潘珠 刘小华 张曼碧 梁秋光 杨柳 曾敏 

机构地区：[1]广东省湛江市鼠疫防治研究所,广东湛江524037

出　　处：《疾病预防控制通报》2013年第2期43-44,共2页Bulletin of Disease Control & Prevention(China)

摘　　要：目的应用PCR试验对广东省3种耶尔森氏菌进行鼠疫的特异性基因检测。方法应用含内部对照PCR技术,以鼠疫菌特异基因fra、pla的2对引物进行PCR实验。结果假结核菌1株、11株小肠结肠炎菌O∶3、5株O∶9、1株中间型,结果全为阴性。结论该试验菌没有鼠疫菌的特异性基因fra、pla,可以进一步说明从1973～1986年雷州半岛用鼠疫IHA、RIHA从鼠类检出的阳性材料是特异的。Objective To detect special gene of three types of Yersinias in Guangdong by PCR assay.Methods Special genes of Yersinias such as fra and pla were chosen as the target genes for PCR assay with inner contrast.Results One strain of Y.pserdotubercolosis,11 strains of Y.enterocolitica O∶3,5 strains of Y.enterocolitica O∶9 and 1 strain of Y.intermedia were all negative.Conclusions The three types of Yersinia had no the special gene of Yersinias.It proves that the positive result is peculiar by IHA and RIHA in plague survey in Leizhou Peninsula from 1973 to 1986.

关 键 词：假结核菌 小肠结肠炎菌 中间型 耶尔森氏菌 fra pla基因 

分 类 号：R378.61[医药卫生—病原生物学] R446[医药卫生—基础医学]