氟硅唑对白术白绢病菌抑菌机制的初步研究  

The Antifungal Mechanism of Flusilazole to Sclerotium rolfsii Sacc. of Atractylodes macrocephala

作　　者：肖仲久[1] 李小霞[1] 邹钦[1] 

机构地区：[1]遵义师范学院生命科学学院,贵州省赤水河流域植物资源保护与应用研究特色重点实验室,贵州遵义563002

出　　处：《农药》2013年第6期439-441,共3页Agrochemicals

基　　金：贵州省遵义市科技支撑计划(社会发展攻关)项目(遵市科合社字2009-24号);贵州省科技厅自然科学基金项目(黔科合J字[2009]2104号);遵义师范学院博士启动基金项目(2012BSJJ09)

摘　　要：[目的]探求40%氟硅唑乳油对白术白绢病菌的抑制机制。[方法]以白术白绢病菌为供试菌,研究该药剂对白术白绢病菌菌丝生长及菌体可溶性蛋白、还原糖、几丁质酶、几丁糖含量的影响。[结果]药剂处理后,白术白绢病菌菌丝严重枯萎变形、内含物渗漏等现象出现;处理24 h,发现菌体可溶性蛋白、还原糖、几丁质酶、几丁糖含量短期内上升随后下降,但随时间变化趋势不同,基本上均高于对照组。[结论]该药剂能够破坏白术白绢病菌细胞的完整性,抑制白术白绢病菌生长。[Aims] The mechanism of flusilazole 40% EC against Sclerotium rolfsii Sacc. of Atractylodes macrocephala was studied. [Mothods] S. rolfsii Sacc. was treated with flusilazole 40% EC, then the hypha growth, soluble protein, reducing sugar, chitinase and chitosan of S. rolfsii Sacc. were determined. [Results] The results showed that the hyphal was withered, deformed seriously and the hyphal cell inclusions leakage constantly. Then the contents of soluble protein, reducing sugar, chitinase and chitosan increased initially and then decline in the short term after 24 h, although the contents trends changed with time, the results of treatment groups were higher than that of control group. [Conclusions] The medicament could destroy the integrity of the S. rolfsii Sacc. cell of A. macrocephala koidz, and constrain the growth ofS. rolfsii Sacc.

关 键 词：氟硅唑 罗氏小核菌 白术 抑菌机制 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]