基于巯基嘌呤/多肽修饰的金纳米粒子的比色方法检测溶液中的镉离子  

A Simple Colorimetric Assay for Detection of Cadmium Ion Based on 6-Mercaptopurine/peptide Functionalized Gold Nanoparticles

作　　者：朱志军[1,2] 刘殿骏[1] 王振新[1] 

机构地区：[1]中国科学院长春应用化学研究所,电分析国家重点实验室,长春130022 [2]中国科学院大学,北京100039

出　　处：《应用化学》2013年第6期716-722,共7页Chinese Journal of Applied Chemistry

基　　金：吉林省科技厅科技发展计划基金(20100701)资助项目

摘　　要：研究了一种基于双配体(巯基嘌呤(MP)和多肽CALNN)修饰金纳米粒子(AuNPs)的比色方法,用于快速、选择性地检测水溶液中的Cd2+。其中,MP作为功能配体通过N原子与Cd2+发生配合作用,从而引起AuNPs聚集;CALNN配体有助于提高体系的稳定性和选择性。当体系中无Cd2+时,溶液呈红色,随着Cd2+浓度的增加,溶液颜色逐渐由红色变为蓝紫色,这种颜色变化可以通过光谱测定还可以用肉眼直接观察。该方法操作简便,具有较好的选择性和较快的响应速度(<5 min),其检测限达到350 nmol/L。A simple colorimetric assay for rapid and selective sensing toxic heavy metal ion, cadmium ion（Cd2＋）, has been developed using gold nanoparticles（AuNPs） decorated with 6-mercaptopurine（MP） and peptide CALNN. MP is employed as a chelating ligand through the N group at the end of MP, and CALNN is served not only as a stabilizer, but also as a factor of high selectivity. AuNPs solution exhibits a gradual color change from red to blue with increasing the concentration of Cd2＋, which leads to an easily measurable change in absorption spectrum of AuNPs solution and naked eye detection. A simple assay with high selectivity and fast response（within 5 min） is presented, and the limit of detection（LOD） for Cd2＋ was calculated to be 350 nmol/L.

关 键 词：金纳米粒子 比色法 Cd2+ 巯基嘌呤 

分 类 号：O652[理学—分析化学]