腹腔液白介素-6、转化生长因子-β和基质金属蛋白酶-7与子宫内膜异位症的相关研究  

Relationship of IL-6, TGF-β, matrix metalloproteinase-7 in peritoneal fluid and endometriosis

作　　者：陈秋梅[1] 郭华[1] 黄艳萍[1] 

机构地区：[1]深圳市宝安区妇幼保健院妇科,广东省深圳518133

出　　处：《中国基层医药》2013年第9期1310-1312,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的 探讨腹腔液中白细胞介素-6（IL-6）、转化生长因子-β（TGF-β）和基质金属蛋白酶-7（MMP-7）在不同r-AFS分期的子宫内膜异位症（EMs）患者腹腔液中的表达。方法 收集80例行腹腔镜检查及治疗的患者腹腔液,其中35例为无子宫内膜异位症的对照组,36例为子宫内膜异位症Ⅰ、Ⅱ期患者,9例为子宫内膜异位症Ⅲ、Ⅳ期的患者。采用双抗体夹心酶联免疫吸附（ELISA）法检测腹腔液中IL-6、TGF-β和MMP-9的浓度,并进行相关性分析。结果 子宫内膜异位症患者腹腔液中IL-6、TGF-β和MMP-7表达分别为（736.5±526.6）ng/L、（936.5±523.5）ng/L和（5.63±1.98）μg/L,明显高于对照组,差异均有统计学意义（均P〈0.01）。子宫内膜异位症Ⅲ、Ⅳ期IL-6和MMP-7浓度分别为（948.6±485.4）ng/L、（8.69±1.23）μg/L,高于Ⅰ、Ⅱ期（453.6±256.8）ng/L、（4.23±1.08）μg/L,差异有统计学意义（均P〈0.05）。IL-6与MMP-7的表达呈显著正相关（r=0.789,P〈0.01）。TGF-β与MMP-7的表达呈显著负相关（r=－0.458,P〈0.05）。结论 子宫内膜异位症患者腹腔液中IL-6、TGF-β、MMP-7可能导致腹腔微环境免疫失衡,并对子宫内膜异位症的发生发展起一定的促进作用。Objective To evaluate the expression of IL-6,TGF-β,matrix metalloproteinase-7 （MMP-7） in the peritoneal fluid of different stage of endometriosis（EMs） patients.Methods Peritoneal fluid samples from female without EMs （n=35） and 45 patients with EMs （36 cases with stageⅠ,Ⅱ;9 cases with stage Ⅲ,Ⅳ）were obtained for detection of IL-6,TGF-β,MMP-7 by ELISA.Results The concentration of IL-6,TGF-β,MMP-7 was （736.5±526.6）ng/L,（936.5±523.5）ng/L and （5.63±1.98）μg/L in EMs patients,which were higher than those in control group （P〈0.01）.The concentration of IL-6,MMP-7 was （948.6±485.4）ng/L,（8.69±1.23）μg/L in stageⅢ,Ⅳgroup,and （453.6±256.8）ng/L,（4.23±1.08）μg/L in stageⅠ,Ⅱgroup,there were significant differences between the two groups （P〈0.05）.The concentration of MMP-7 was positively correlated with IL-6（r=0.789,P〈0.01）,and was negatively correlated with TGF-β（r=-0.458,P〈0.05）.Conclusion IL-6,TGF-β,MMP-7 may play roles in microecology immune imbalance and the development of EMs.

关 键 词：子宫内膜异位症 腹腔灌洗 基质金属蛋白酶-7 白细胞介素-6 转化生长因子-Β 

分 类 号：R711.71[医药卫生—妇产科学]