96份小麦品种资源抗白粉病基因的分子检测  

Detection of Powdery Mildew Resistance Genes in 96 Wheat Variety Resources Using Molecular Markers

作　　者：王玉叶[1,2] 张海萍[1] 潘艳秋[1] 常成[1] 马传喜[1] 

机构地区：[1]安徽农业大学农学院,合肥230036 [2]安徽省淮南农场农科所,安徽淮南232035

出　　处：《种子》2013年第5期20-24,共5页Seed

基　　金：现代农业产业技术体系(编号:nycytx-03);国家科技支撑计划(编号:2009BADA6B01)

摘　　要：小麦白粉病已成为当前小麦生产的严重威胁之一,加快白粉病抗性基因在小麦育种及生产中的应用具有重要的意义。利用前人已开发的Pm 21、Pm 30、Pm 34的STS、SCAR和SSR标记对96份小麦品种资源进行检测。结果表明,96份材料中,4份材料含有Pm 21基因(占4.17%)、9份材料含有Pm 30基因(占9.37%)、21份材料含有Pm 34基因(占21.87%);其中1份材料以Pm 21+Pm 30(占1.04%)聚合形式存在;另1份材料以Pm 30+Pm 34(占1.04%)聚合形式存在;没有发现Pm 21+Pm 34聚合类型以及Pm 21+Pm 30+Pm 34聚合类型。上述结果可以为小麦抗病育种中抗病亲本的选配提供参考信息。Accelerating application of the powdery mildew resistance genes in wheat breeding and production has important significance because wheat powdery mildew has become one of the serious threat to wheat production. In this article,we detected the distribution of Pm 21, Pm 30, and Pm 34 genes in 96 wheat variety resources using STS, SCAR and SSR molecular markers. The results showed that the frequency of Pm 34 gene （21 wheat varieties,21.87% ） was highest,Pro 30 was higher （9 wheat varieties,9.37% ） and Pm 21 was lowest （4 wheat varieties,4.17% ）in 96 wheat variety resources. What＇ s more ,the research showed that part of wheat variety resources contained two kinds of resistance genes combination. One material （ 1.04% ） was detected to contain Pm 21 ＋ Pm 30. The other （ 1.04% ） was detected to contain Pm 30 ＋ Pm 34. We did not find Pm 21 ＋ Pm 34 or Pm 21 ＋ pm 30 ＋ Pm 34 combination type. These results may provide reference informa- tion for preparing disease-resistant parents in wheat disease resistance breeding.

关 键 词：小麦 白粉病 分子标记 

分 类 号：S512.1[农业科学—作物学]