高效液相色谱法测定山茱萸中熊果酸与齐墩果酸的含量  

Content Determination of Ursolic Acid and Oleanolic Acid in Comus Officinalis by High Performance Liquid Chromatography

作　　者：刘毅[1,2] 金传山[3] 许甜甜[3] 

机构地区：[1]安徽中医药大学研究生部,安徽合肥230038 [2]安庆医药高等专科学校,安徽安庆246052 [3]安徽中医药大学药学院,安徽合肥230031

出　　处：《安徽中医学院学报》2013年第3期85-87,共3页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的采用高效液相色谱法同时测定山茱萸中熊果酸与齐墩果酸的含量,为山茱萸的质量控制提供依据。方法 Diamonsil-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-(水∶冰乙酸∶三乙胺(30∶0.1∶0.05))(88∶12),柱温25℃,流速1.0ml/min,进样量20μl,检测波长为210nm。结果熊果酸在0.031 5～0.315mg/ml,齐墩果酸在0.015 6～0.156mg/ml的浓度范围内与峰面积呈良好的线性关系;熊果酸和齐墩果酸的平均回收率分别为97.53%、100.08%,RSD分别为1.28%、1.98%。结论本法操作简单,重复性好,结果准确可靠,可用于山茱萸的质量控制。Objective To determine the content of ursolic acid and oleanolic acid in Comus officinalis by length of 210 nm. The mobile phase used in HPLC consisted of water and a mixture （composed of water, glacial acetic acid, and triethylamine at a ratio of 30：0.1：0.05） at a ratio of 88：12. Results Ursolic acid and oleanolic acid had a good linear relationship between concentration and peak area in the concentration ranges of 0. 031 5-0. 315 mg/ml and 0. 015 6-0. 156 mg/ml, respectively; the average recovery rates of ursolic acid and oleanolic acid were 97.53% （RSD = 1.28%） and 100. 08% （RSD - 1.98%）, respectively. Conclusion This HPLC method is easy to operate, highly repeatable, accurate, and reliable and can be used for the quality control of Comus officinalis.

关 键 词：山茱萸 熊果酸 齐墩果酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]