羰基生物还原法合成手性醇的研究进展  

Advances in synthesis of chiral alcohols by carbonyl bioreduction

作　　者：郁惠蕾[1] 黄磊[1] 倪燕[1] 许国超[1] 许建和[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物加工过程》2013年第3期71-82,共12页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(20902023)

摘　　要：手性醇是合成许多光学活性药物、农用化学品及其他精细化学品的关键手性砌块。羰基生物还原法理论上可实现100%转化率,且反应条件温和,对环境十分友好,被普遍认为是生产手性醇的绿色、高效途径。综述了近年来利用生物信息学、高通量筛选和蛋白质工程的发展对新型、高效生物催化剂开发的影响,特别是利用相关技术手段开发羰基还原酶的进展。Enantiopure secondary alcohols, as key intermediates, play an essential role in pharmaceutical, agrochemical, and chemical industries. Biocatalytic ketone reduction is being generally regarded as a green and effective access to enantiopure alcohols, due to mildness of reaction conditions and friendliness to environment. An increasing number of novel and robust enzymes become more easily accessible as a result of the ongoing progress in genomics, high-throughput screening, and directed evolution technologies. Advanced bioprocess engineering provide more opportunities for applying enzyme technologies to meet chiral challenges in synthetic chemistry.

关 键 词：不对称还原 生物催化 羰基还原酶 手性醇 基因组挖掘 

分 类 号：Q939.9[生物学—微生物学]