幽门螺杆菌培养方法的改良和鉴定  

Improvement and identification of the method of culturing helicobacter pylori

作　　者：刘代华[1,2] 罗宜辉[1,3] 梁健成[1,2] 练海燕[1,3] 李运泽[1,3] 黄义昆[1,2] 

机构地区：[1]广西医科大学第五附属医院 [2]柳州市人民医院药剂科,广西柳州545006 [3]柳州市人民医院消化内科,广西柳州545006

出　　处：《华夏医学》2013年第1期58-61,共4页Acta Medicinae Sinica

基　　金：广西柳州市应用技术研究与开发计划课题(2010030711);广西壮族自治区卫生厅自筹经费科研课题(22010053)

摘　　要：目的:在我院建立简易的幽门螺杆菌培养方法。方法:胃镜下取患者胃黏膜,尿素酶试剂盒检测幽门螺杆菌为阳性者进行培养,在微需氧环境中培养5d,取原代传代。原代菌株用尿素酶法、过氧化氢酶法、氧化酶法和革兰染色鉴定,同时符合者为幽门螺杆菌。结果:53例胃黏膜标本培养,45例培养出幽门螺杆菌,成功率为84.91%,通过以上方法鉴定符合幽门螺杆菌标准。结论:建立了简单有效的培养幽门螺杆菌的方法。Objective： To establish a simple method of culturing H elicobacter pylori （H pylori） in our hospital. Methods： The gastric mucosa taken under gastroscope and tested positive by urease test were cultured in microaerophilic environment for 5 days, and the primary bacterias were passaged. The primay bacterias were identified by urease test, catalase test, oxidase test and Gram staini All the results accorded with H pylori were considered successful. Results： Of the culture of ng. the gastric mucosa in 53 cases, 45 specimen were successful to develop the standard H pylori deter- mined by the aboved test methods, which was of the success rate of 84.91%. Conclusion： Simple and effective method to culture helieobacter pylori was constructed.

关 键 词：幽门螺杆菌 培养 鉴定 微需氧 

分 类 号：R378.2[医药卫生—病原生物学]