灯盏细辛干预金地鼠颊囊癌变过程的α-SMA动态观察  

Observation on α-SMA during Erigeron Breviscapus(Vant) Hand-Mazz Obstructs the Evolution of Carcinogenesis of Gloden Hamster Cheek Pouch

作　　者：周曾同[1] 张水龙[1] 丁瑞宇 华丽[1] 钟文静[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔内科,上海200011

出　　处：《上海口腔医学》2000年第2期107-109,共3页Shanghai Journal of Stomatology

基　　金：上海市科委基金!(96411900)

摘　　要：目的　动态观察灯盏细辛 (HEr)对金地鼠颊囊白斑癌变过程中α 平滑肌肌动蛋白 (α SMA)表达规律的影响 ,探讨HEr抗白斑癌变的可能机理。方法　金地鼠 10 2只随机分为模型组和HEr组 ,各用灯盏细辛流浸膏干预二甲基苯丙蒽 (DMBA)诱导的金地鼠白斑癌变进程。免疫组化技术检测涂布DMBA 4 9周的颊囊标本α SMA表达水平 ,并与模型组对照。结果　α SMA连续性染色血管密度平均值HEr组为 6 5 .76 ,模型组为 42 .12 (P <0 .0 0 1)。按10 0 %、5 0 %、2 0 %、10 %、3% 5级分档 ,HEr组的高分级例数远多于模型组 (P <0 .0 1)。结论　HEr有上调金地鼠白斑癌变过程中α SMA表达水平的确切作用 。Objective To observe dynamically that Erigeron Breviscapus (Vant) Hand-Mazz (HEr) affects the expression of α-smooth muscle actin (α-SMA).To discuss the probable mechanism of obstructing leukoplakia carcinogenesis of this medicine. Methods 120 golden hamsters were randomly divided into model group (48), HEr group (48) and control group (6). HEr was applied to obstruct the evolution of carcinogenesis of golden hamster cheek pouch. Immunohistochemistry was used to detect the expression level of α-SMA with cheek pouch specimen that besmears DMBA in 4-9 weeks.Results were compared with model group. Results Vessel density dyed with αSMA continuously of HEr group was 65.76 significantly higher than that of model group 42.12 (P<0.001). High classification cases in HEr group were much more than model group when cases were divided into five groups as follow: 100%, 50%, 20%, 10%, 3% (P<0.01). Conclusion HEr can raise the expression level of αSMA exactly during the evolution of leukoplakia carcinogenesis of golden hamster,which shows that this medicine obstructs carcinogenesis by keeping the normal physiological function of vascular myoepithelial cell and integrity of vascular basement membrane. [

关 键 词：灯盏细辛 Α-平滑肌肌动蛋白 白斑 癌变 

分 类 号：R285[医药卫生—中药学]