大孔吸附树脂法同时纯化银杏叶中黄酮苷和萜类内酯  

Study on simultaneous purification of ginkgo flavone glycosides and terpene lactones from Ginkgo biloba L.Leaves by macroporous adsorption resin

作　　者：钦富华[1] 黄孝闻[2] 夏晓静[1] 吴人杰[2] 王明军[1] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]浙江省中医药研究院,浙江杭州310007

出　　处：《广东药学院学报》2013年第2期142-146,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：宁波市工业科研攻关项目(2007B10013)

摘　　要：目的探讨大孔吸附树脂同时纯化银杏叶黄酮苷和萜类内酯的工艺条件。方法对D101、X-5、S-8、D4020、HP-20、AB-8 6种大孔吸附树脂进行筛选,然后对最佳上样量、除杂洗脱条件、洗脱剂乙醇体积分数及洗脱剂用量等因素进行了优化。结果 D101型大孔树脂分离纯化效果最佳。优化工艺条件为:上样液生药质量与树脂质量比为1∶1,20%(体积分数)乙醇作为除杂溶剂,洗脱体积为5 BV,60%(体积分数)乙醇作为洗脱液,洗脱体积为4 BV。纯化物中黄酮苷和萜类内酯平均质量分数分别为24.93%和7.36%。结论 D101大孔吸附树脂对银杏叶黄酮苷和萜类内酯的综合吸附性能较好,适用于银杏叶提取物的分离纯化。Objective To investigate the simultaneous purification of flavone glycosides and terpene lactones from Ginkgo biloba L.Leaves by macroporous adsorption resin.Methods Six types of resins were screened,including D101,X-5,S-8,D4020,HP-20 and AB-8,and the optimum sample size,eluting amount for removing impurities,the eluent′s concentration and amount of ethanol were optimized.Results D101 resin was suitable for the simultaneous purification of flavone glycosides and terpene lactones.The optimized conditions were as follows： the proportion of crude drug and resin was 1∶1,the volume of 20% ethanol was 5 BV,and the volume of 60% ethanol as the eluting agent was 4 BV.The purity of flavone glycosides and terpene lactones from purification were 24.93% and 7.36%,respectively.Conclusion D101 resin showed good comprehensive adsorption property,and it was suitable for the separation and purification of Ginkgo biloba extract（GBE）.

关 键 词：银杏叶 大孔吸附树脂 纯化 黄酮苷 萜类内酯 

分 类 号：R284.2[医药卫生—中药学]