超声波提取与大孔树脂富集佛甲草总黄酮的工艺研究  

Study on Ultrasonic Extraction and Macroporous Resin Purification Techniques of Total Flavones from Sedum lineare

作　　者：苏建伟[1] 贾志敏[1] 谢扬[1] 

机构地区：[1]南方医科大学药学院,广东广州510515

出　　处：《中药材》2013年第2期298-301,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技重大专项(2009ZX09103-009)

摘　　要：目的:优选超声波法提取佛甲草总黄酮工艺,研究大孔树脂富集纯化佛甲草总黄酮的工艺条件。方法:采用正交设计法考察乙醇浓度、料液比、超声时间和超声温度对佛甲草总黄酮提取率的影响,以佛甲草总黄酮的吸附量和解析率为评价指标,考察富集纯化佛甲草总黄酮大孔树脂的吸附性能和洗脱参数。结果:超声提取佛甲草总黄酮的最佳工艺为:乙醇浓度80%、料液比1∶40、超声时间20 min、超声温度30℃。采用AB-8型大孔树脂富集纯化佛甲草总黄酮效果最佳洗脱条件为:上样浓度为1.59 mg/mL、80%乙醇为洗脱剂,洗脱速度为2 mL/min,洗脱率达94.72%,总干燥物中佛甲草总黄酮的含量为43.51%。结论:超声波法联用AB-8型大孔树脂提取纯化佛甲草总黄酮方法简单,易行,生产成本低。Objective：To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin.Methods：Optimum ultrasonic extraction conditions（ethanol concentration,solid/liquid,ultrasonic time,working temperature）were obtained through orthogonal design.The adsorption ratio and eluting ratio of total flavones were selected as indexes.Results：The optimum ultrasonic extraction condition was 80% ethanol,solid/liquid 1∶ 40,ultrasonic 20 min at 30 ℃.The adsorbed AB-8 macroporous resin column was eluted by 80% ethanol at the eluting velocity of 2 mL/min,the eluting ratio and purity of total flavones was 94.72% and 43.51%,respectively.Conclusion：The method is simple and low cost.

关 键 词：佛甲草 总黄酮 超声提取 大孔树脂 

分 类 号：R284.2[医药卫生—中药学]